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Literature summary for 2.7.1.77 extracted from

  • Prasher, D.C.; Carr, M.C.; Ives, D.H.; Tsai, T.C.; Frey, P.A.
    Nucleoside phosphotransferase from barley. Characterization and evidence for ping pong kinetics involving phosphoryl enzyme (1982), J. Biol. Chem., 257, 4931-4939.
    View publication on PubMed

General Stability

General Stability Organism
withstands repeated freezing and thawing if the protein concentration is less than 0.5 mg/ml Hordeum vulgare

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 2 mM, at least 80% inhibition of transphosphorylation Hordeum vulgare
Cd2+ 2 mM, at least 80% inhibition of transphosphorylation Hordeum vulgare
Cu2+ 2 mM, at least 80% inhibition of transphosphorylation Hordeum vulgare
EDTA 2 mM, 85% inhibition. 95% inhibition at 10 mM Hordeum vulgare
Hg2+ 2 mM, at least 80% inhibition of transphosphorylation Hordeum vulgare
Zn2+ 2 mM, 20% inhibition of transphosphorylation Hordeum vulgare

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.087
-
deoxyadenosine pH 5.0, reaction with AMP Hordeum vulgare
0.412
-
2'-deoxyguanosine pH 5.0, reaction with AMP Hordeum vulgare
0.89
-
2'-deoxythymidine pH 5.0, reaction with AMP Hordeum vulgare
2.13
-
guanosine pH 5.0, reaction with AMP Hordeum vulgare
3.93
-
uridine pH 5.0, reaction with AMP Hordeum vulgare
4.15
-
2'-deoxycytidine pH 5.0, reaction with AMP Hordeum vulgare
6.39
-
cytidine pH 5.0, reaction with AMP Hordeum vulgare

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement Hordeum vulgare
Co2+ 2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement Hordeum vulgare
Mg2+ 2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement Hordeum vulgare
Mg2+ 2 mM, with 0.5 mM AMP and deoxythymidine, 50% stimulation Hordeum vulgare

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
2 * 50000, SDS-PAGE Hordeum vulgare
53400
-
non-denaturing PAGE Hordeum vulgare

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein carrying a variable amount of polysaccharide Hordeum vulgare

Purification (Commentary)

Purification (Comment) Organism
-
Hordeum vulgare

Reaction

Reaction Comment Organism Reaction ID
a nucleotide + a 2'-deoxyribonucleoside = a nucleoside + a 2'-deoxyribonucleoside 5'-phosphate ping pong kinetics Hordeum vulgare

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
Hordeum vulgare
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
180
-
-
Hordeum vulgare

Storage Stability

Storage Stability Organism
-20°C, stable for at least 6 months Hordeum vulgare
0°C, 0.01% Triton X-100 or 0.25 M sodium acetate, pH 5, 44 h, phosphotransferase activity decreases 20% and 50% respectively Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-AMP + 2'-deoxycytidine
-
Hordeum vulgare dCMP + adenosine
-
?
3'-AMP + 2'-deoxyguanosine
-
Hordeum vulgare dGMP + adenosine
-
?
3'-AMP + 2'-deoxythymidine
-
Hordeum vulgare dTMP + adenosine
-
?
3'-AMP + cytidine
-
Hordeum vulgare CMP + adenosine
-
?
3'-AMP + guanosine
-
Hordeum vulgare GMP + adenosine
-
?
4-nitrophenyl phosphate + 2'-deoxyadenosine
-
Hordeum vulgare 4-nitrophenol + 2'-deoxyadenosine 5'-phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 50000, SDS-PAGE Hordeum vulgare

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Hordeum vulgare

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
phosphatase activity Hordeum vulgare
6.5
-
transferase activity Hordeum vulgare

pH Range

pH Minimum pH Maximum Comment Organism
4.5 8.5 pH 4.5: about 65% of maximal activity, pH 8.5: about 50% of maximal activity Hordeum vulgare