Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetone | 25%, 2.45fold stimulation | Hordeum vulgare | |
acetonitrile | 8%, 2.4fold stimulation | Hordeum vulgare | |
Dimethyl formamide | 5%, 1.8fold, stimulation | Hordeum vulgare | |
dimethyl sulfoxide | 20%, 1.8fold stimulation | Hordeum vulgare | |
glycerol | 60%, 1.7fold stimulation | Hordeum vulgare | |
methanol | 30%, 2.3fold stimulation | Hordeum vulgare |
General Stability | Organism |
---|---|
storage in 20% acetone for 1 week without loss of activity | Hordeum vulgare |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arsenate | strong competitive inhibitor | Hordeum vulgare | |
cAMP | strong competitive inhibitor | Hordeum vulgare | |
EDTA | cAMP and arsenate can reduce the rate of inactivation | Hordeum vulgare |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can restore activity of the metal-free apoenzyme | Hordeum vulgare | |
Cu2+ | can restore activity of the metal-free apoenzyme to a minor extent | Hordeum vulgare | |
Fe2+ | can restore activity of the metal-free apoenzyme to a minor extent | Hordeum vulgare | |
Mg2+ | contains one Mg2+ per dimeric enzyme molecule, the metal-free apoenzyme is inactive | Hordeum vulgare | |
Ni2+ | can restore activity of the metal-free apoenzyme to a minor extent | Hordeum vulgare | |
Zn2+ | can restore activity of the metal-free apoenzyme to a minor extent | Hordeum vulgare |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
sprout | - |
Hordeum vulgare | - |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermal stability is reduced when metal is removed but can be restored by addition of Mg2+ | Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Hordeum vulgare |