Activating Compound | Comment | Organism | Structure |
---|---|---|---|
polyamines | stimulate at suboptimal Mg2+ concentration | Bos taurus | |
spermidine | 3-4fold activation, activates by lowering the Km for Mg2+ and enhancing Vmax, optimal at 10-15 mM | Bos taurus | |
spermine | activates 3-4fold at 2 mM | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ammonium sulfate | about 75% inhibition at 10 mM | Bos taurus | |
diphosphate | weak | Bos taurus | |
p-chloromercuribenzoate | reversible | Bos taurus | |
sulfate | weak | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
ATP | pH 5.5, 37°C | Bos taurus | |
2.5 | - |
Mg2+ | pH 5.5, 37°C | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | KCl slightly stimulates | Bos taurus | |
Mg2+ | required | Bos taurus | |
Mg2+ | optimal concentration is 10 mM | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
1 * 54000, SDS-PAGE | Bos taurus |
56000 | - |
gel filtration | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-dephospho-DNA | Bos taurus | - |
ADP + 5'-phospho-DNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
calf | - |
Purification (Comment) | Organism |
---|---|
to homogeneity | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
thymus | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0167 | - |
purified enzyme | Bos taurus |
Storage Stability | Organism |
---|---|
0°C, stable for at least 1 month | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-dephospho-DNA | - |
Bos taurus | ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-RNA | no activity | Bos taurus | ADP + 5'-phospho-RNA | - |
? | |
ATP + synthetic oligonucleotide | no activity with poly(A) | Bos taurus | ADP + oligonucleotide 5'-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 54000, SDS-PAGE | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 30 | - |
Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
sharp maximum | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | - |
diphosphate | pH 5.5, 25°C | Bos taurus | |
7 | - |
sulfate | pH 5.5, 25°C | Bos taurus |