Application | Comment | Organism |
---|---|---|
synthesis | production of beta,gamma-imidoadenylyl 5'-tetraphosphate by using beta,gamma-imidoadenylyl 5'-triphosphate as substrate in the reverse reaction | Tequatrovirus T4 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta,gamma-imidoadenylyl 5'-tetraphosphate | ATP analog, competitive against ATP, noncompetitive against 5'-OH-DNA, serves as substrate for the reverse reaction only | Tequatrovirus T4 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Tequatrovirus T4 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-dephospho-DNA | Tequatrovirus T4 | - |
ADP + 5'-phospho-DNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-dephospho-DNA | - |
Tequatrovirus T4 | ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-DNA | - |
Tequatrovirus T4 | ADP + 5'-phospho-DNA | - |
r | |
ATP + synthetic oligonucleotide | oligo dpT(pT)9 | Tequatrovirus T4 | ADP + oligonucleotide 5'-phosphate | - |
? | |
ATP + synthetic oligonucleotide | oligodeoxynucleotides of chain length less than approximately 10-12 residues | Tequatrovirus T4 | ADP + oligonucleotide 5'-phosphate | - |
? | |
beta,gamma-imidoadenylyl 5'-triphosphate + 5'-phospho-DNA | ATP analog, inhibitory, competitive against ATP | Tequatrovirus T4 | beta,gamma-imidoadenylyl 5'-tetraphosphate + 5'-dephospho-DNA | - |
ir | |
beta,gamma-imidoadenylyl 5'-triphosphate + 5'-phospho-DNA | is no substrate in the forward reaction but can replace ADP and ATP in the reverse reaction | Tequatrovirus T4 | beta,gamma-imidoadenylyl 5'-tetraphosphate + 5'-dephospho-DNA | - |
ir |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Tequatrovirus T4 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Tequatrovirus T4 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | Tequatrovirus T4 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
beta,gamma-imidoadenylyl 5'-tetraphosphate | versus ATP, pH 8.0, 35°C, independent of pH-value | Tequatrovirus T4 | |
11 | - |
beta,gamma-imidoadenylyl 5'-tetraphosphate | versus 5'-OH-DNA, pH 8.0, 35°C, independent of pH-value | Tequatrovirus T4 |