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Literature summary for 2.7.1.78 extracted from

  • Soltis, D.A.; Uhlenbeck, O.C.
    Isolation and characterization of two mutant forms of T4 polynucleotide kinase (1982), J. Biol. Chem., 257, 11332-11339.
    View publication on PubMed
Search for more literature for 2.7.1.78

Protein Variants

Protein Variants Comment Organism
additional information natural mutants pseT 1 and pseT 47, the first shows no 3'-phosphatase activity, but normal polynucleotide kinase activity, the second shows very little 3'-phosphatase activity, but no polynucleotide kinase activity, expression in Escherichia coli Tequatrovirus T4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0027
-
 5'-OH-DNA wild-type, pH 7.6, 37°C Tequatrovirus T4
0.0031
-
 5'-OH-DNA mutant pseT 1, pH 7.6, 37°C Tequatrovirus T4
0.0045
-
 5'-OH-DNA mutant pseT 47, pH 7.6, 37°C Tequatrovirus T4
0.152
-
 ATP mutant pseT 1, pH 7.6, 37°C Tequatrovirus T4
0.171
-
 ATP mutant pseT 47, pH 7.6, 37°C Tequatrovirus T4
0.188
-
 ATP wild-type, pH 7.6, 37°C Tequatrovirus T4

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 5'-dephospho-DNA Tequatrovirus T4
-
 ADP + 5'-phospho-DNA
-
 ?

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
 from infected Escherichia coli
-
Tequatrovirus T4
-
 mutant forms, genes pseT 1 and pseT 47
-
Tequatrovirus T4
-
 gene pseT
-

Purification (Commentary)

Purification (Comment) Organism
2 mutant forms pseT 1 and pseT 47 Tequatrovirus T4

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.67
-
 purified wild-type Tequatrovirus T4
7
-
 purified mutant pseT 1 Tequatrovirus T4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5'-dephospho-DNA
-
 Tequatrovirus T4 ADP + 5'-phospho-DNA
-
 ?
additional information enzyme also has DNA 3'-phosphatase activity Tequatrovirus T4 ?
-
 ?

Synonyms

Synonyms Comment Organism
More enzyme also possesses a 3'-phosphatase activity Tequatrovirus T4

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Tequatrovirus T4

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Tequatrovirus T4

Cofactor

Cofactor Comment Organism Structure
ATP dependent on Tequatrovirus T4