Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | - |
Homo sapiens | |
dithiothreitol | - |
Homo sapiens | |
sulfhydryl reagents | - |
Homo sapiens |
General Stability | Organism |
---|---|
bovine serum albumin and dithiothreitol stabilize the enzyme during activity assay | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-AMP | weak | Homo sapiens | |
5'-Hydroxyl poly(I) | in combination with 5'-hydroxyl poly(A) or poly(C) | Homo sapiens | |
ADP | - |
Homo sapiens | |
Cu2+ | inhibits in presence of MgCl2 | Homo sapiens | |
diphosphate | - |
Homo sapiens | |
KCl | weak inhibition, 50% at about 0.3 M | Homo sapiens | |
Mn2+ | inhibition above 1 mM | Homo sapiens | |
N-ethylmaleimide | - |
Homo sapiens | |
NaCl | weak inhibition, 50% at about 0.3 M | Homo sapiens | |
NH4+ | 50% at about 0.3 M; weak inhibition | Homo sapiens | |
phosphate | weak | Homo sapiens | |
Ribonucleoside 3'-phosphates | weak | Homo sapiens | |
sulfate | weak | Homo sapiens | |
Zn2+ | inhibition in presence of Mg2+ | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
5'-hydroxyl poly(A) | pH 8.4, 37°C | Homo sapiens | |
0.0034 | - |
5'-hydroxyl poly(C) | pH 8.4, 37°C | Homo sapiens | |
0.5 | - |
ATP | pH 8.4, 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Homo sapiens | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | divalent cation required, slight activation in absence of MgCl2 | Homo sapiens | |
Ca2+ | about 17% of the activity with Mn2+, synergistic to Mn2+ | Homo sapiens | |
divalent cations | absolutely required | Homo sapiens | |
Mg2+ | Mg2+ or Mn2+ effective in activation | Homo sapiens | |
Mg2+ | 86% of the activity with Mn2+ | Homo sapiens | |
Mn2+ | Mg2+ or Mn2+ effective in activation | Homo sapiens | |
Mn2+ | maximal activation at 0.6-1.0 mM, inhibition above 1 mM | Homo sapiens | |
Zn2+ | no activation, inhibition in presence of Mg2+ | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-dephospho-DNA | Homo sapiens | - |
ADP + 5'-phospho-DNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
HeLa cells | - |
Purification (Comment) | Organism |
---|---|
431fold | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | HeLa cells | Homo sapiens | - |
HeLa cell | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.125 | - |
purified enzyme, substrate RNA | Homo sapiens |
Storage Stability | Organism |
---|---|
0°C, 10% loss of activity after 2 months | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-dephospho-DNA | - |
Homo sapiens | ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-RNA | much more efficient as substrate than DNA | Homo sapiens | ADP + 5'-phospho-RNA | - |
? | |
ATP + 5'-hydroxyl poly(A) | - |
Homo sapiens | ? | - |
? | |
ATP + 5'-hydroxyl poly(C) | - |
Homo sapiens | ? | - |
? | |
ATP + synthetic oligonucleotide | 5'-hydroxyl poly(A) | Homo sapiens | ADP + oligonucleotide 5'-phosphate | - |
? | |
ATP + synthetic oligonucleotide | 5'-hydroxyl poly(C) | Homo sapiens | ADP + oligonucleotide 5'-phosphate | - |
? | |
ATP + synthetic oligonucleotide | low activity with 5'-hydroxyl poly(I) | Homo sapiens | ADP + oligonucleotide 5'-phosphate | - |
? | |
ATP + synthetic oligonucleotide | 5'-hydroxyl poly(dA), at 6% of 5'-hydroxyl poly(A) | Homo sapiens | ADP + oligonucleotide 5'-phosphate | - |
? | |
CTP + 5'-dephospho-DNA | CTP gives 15% of the activity with ATP | Homo sapiens | CDP + 5'-phospho-DNA | - |
? | |
CTP + 5'-dephospho-RNA | less effective than ATP | Homo sapiens | CDP + 5'-phospho-RNA | - |
? | |
GTP + 5'-dephospho-DNA | GTP gives 15% of the activity with ATP | Homo sapiens | GDP + 5'-phospho-DNA | - |
? | |
[gamma-S]GTP + 5'-dephospho-RNA | less effective than ATP | Homo sapiens | [gamma-S]GDP + 5'-phospho-RNA | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
57% loss of RNA-dependent activity, 34% of DNA-dependent activity | Homo sapiens |
55 | - |
5 min, complete loss of activity | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at | Homo sapiens |
5.5 | - |
substrate DNA | Homo sapiens |
7.9 | 8.9 | - |
Homo sapiens |
8.4 | - |
assay at, substrate RNA | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.9 | pH 6.5: about 50% of activity maximum, pH 7.9-8.9: activity maximum, substrate RNA | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | Homo sapiens | |
ATP | best at 2 mM | Homo sapiens | |
CDP | - |
Homo sapiens | |
CTP | - |
Homo sapiens | |
GDP | - |
Homo sapiens | |
GTP | - |
Homo sapiens |