Cloned (Comment) | Organism |
---|---|
the PNKP mutants are subcloned into the vector pET16b for expression in Escherichia coli BL21DE3 pLysS cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
W331F | single mutant, 90% phosphatase activity | Homo sapiens |
W402F | single mutant, 85% phosphatase activity | Homo sapiens |
WFX402 | mutant, all tryptophans except 402 are replaced by phenylalanine, 85% phosphatase activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-(1-hydroxyundecyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione | A12B4C3, noncompetitive inhibition | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-dephospho-DNA | Homo sapiens | - |
ADP + 5'-phospho-DNA | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q96T60 | - |
- |
Purification (Comment) | Organism |
---|---|
the mutant proteins are purified using ProBond resin | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
A-549 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-dephospho-DNA | - |
Homo sapiens | ADP + 5'-phospho-DNA | - |
r |
Synonyms | Comment | Organism |
---|---|---|
PNKP | - |
Homo sapiens |
polynucleotide kinase/phosphatase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
inhibition assay | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
inhibition assay | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | effect of A12B4C3 binding on the W331F mutant, 95% inhibition | Homo sapiens | |
additional information | - |
additional information | effect of A12B4C3 binding on the W402F mutant, 6% inhibition | Homo sapiens | |
additional information | - |
additional information | effect of A12B4C3 binding on the WFX402 mutant, 90% inhibition | Homo sapiens | |
additional information | - |
additional information | effect of A12B4C3 binding on the wild-type PNKP, 100% inhibition | Homo sapiens |