Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Amino-5-hydroxyadipate | 4 isomers, 40 mM, strong inhibition | Rattus norvegicus | |
4,5-Dehydro-(trans)-L-lysine | - |
Rattus norvegicus | |
carnitine | 2 isomers | Rattus norvegicus | |
choline | 40 mM, weak inhibition | Rattus norvegicus | |
D-Lysine | - |
Rattus norvegicus | |
D-ornithine | - |
Rattus norvegicus | |
ethanolamine | 40 mM, weak inhibition | Rattus norvegicus | |
Hydroxy-L-pipecolate | - |
Rattus norvegicus | |
hydroxy-L-proline | 40 mM, strong | Rattus norvegicus | |
L-2,4-diamino-n-butyrate | - |
Rattus norvegicus | |
L-homoserine | 40 mM, weak inhibition | Rattus norvegicus | |
L-lysine | higher inhibitory effect than with D-lysine | Rattus norvegicus | |
L-ornithine | higher inhibitory effect than with D-ornithine | Rattus norvegicus | |
Mn2+ | at 1 mM, in presence of 1 mM Mg2+, strong inhibition, but activation in absence of Mg2+ | Rattus norvegicus | |
S-aminoethyl-L-cysteine | - |
Rattus norvegicus | |
Zn2+ | at 1 mM, in presence of 1 mM Mg2+, strong inhibition | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0056 | - |
hydroxy-L-lysine | pH 8.0, 37°C | Rattus norvegicus | |
0.0062 | - |
allohydroxy-L-lysine | pH 8.0, 37°C | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | requirement of a divalent metal ion, 1 mM, 13% of activity compared to Mg2+ | Rattus norvegicus | |
Co2+ | requirement of a divalent metal ion, 1 mM, 13% of activity compared to Mg2+ | Rattus norvegicus | |
Cu2+ | requirement of a divalent metal ion, 1 mM, 13% of activity compared to Mg2+ | Rattus norvegicus | |
Fe2+ | requirement of a divalent metal ion, 1 mM, 13% of activity compared to Mg2+ | Rattus norvegicus | |
Hg2+ | requirement of a divalent metal ion, 1 mM, 13% of activity compared to Mg2+ | Rattus norvegicus | |
Mg2+ | requirement of a divalent metal ion, Mg2+ yields highest activity | Rattus norvegicus | |
Mn2+ | requirement of a divalent metal ion, 1 mM, 13% of activity compared to Mg2+, strong inhibition in presence of 1 mM Mg2+ | Rattus norvegicus | |
additional information | K+, Li+ have no influence | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + 5-hydroxy-L-lysine | Rattus norvegicus | erythro-5-hydroxy-L8-lysine | GDP + O-phosphohydroxy-L-lysine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Cebus albifrons | - |
- |
- |
Chlorocebus aethiops | - |
- |
- |
Gallus gallus | - |
- |
- |
Mus musculus | - |
- |
- |
no activity in Homo sapiens | - |
no activity in liver and kidney | - |
Oryctolagus cuniculus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
1200fold | Rattus norvegicus |
partial | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Mus musculus | - |
kidney | - |
Rattus norvegicus | - |
kidney | - |
Oryctolagus cuniculus | - |
kidney | - |
Chlorocebus aethiops | - |
kidney | - |
Cebus albifrons | - |
liver | - |
Gallus gallus | - |
liver | - |
Mus musculus | - |
liver | - |
Rattus norvegicus | - |
liver | - |
Bos taurus | - |
liver | - |
Oryctolagus cuniculus | - |
liver | - |
Cebus albifrons | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.114 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
frozen, little loss of activity for weeks | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + 5-hydroxy-L-lysine | erythro-5-hydroxy-L8-lysine | Rattus norvegicus | GDP + O-phosphohydroxy-L-lysine | - |
? | |
GTP + allohydroxy-L-lysine | - |
Rattus norvegicus | ? | - |
? | |
additional information | no activity with D-isomers, ITP can partially substitute for GTP | Rattus norvegicus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | 25% of maximal activity at pH 6.0, 35% of maximal activity at pH 9.0 | Rattus norvegicus |