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Literature summary for 2.7.1.90 extracted from
- Isolation and characterization of pyrophosphate:D-D-fructose-6-phosphate 1-phosphotransferase from cucumber seeds (1986), Plant Cell Physiol., 27, 1285-1295.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose 2,6-bisphosphate | - |
Cucumis sativus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | noncompetitive versus diphosphate and D-fructose 6-phosphate; sequential ternary complex mechanism | Cucumis sativus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | less effective than Mg2+ | Cucumis sativus | |
| divalent cations | required | Cucumis sativus | |
| divalent cations | descending order Mg2+, Mn2+ and Co2+ | Cucumis sativus | |
| divalent cations | Mn2+ or Mg2+ | Cucumis sativus | |
| Mg2+ | requirement | Cucumis sativus | |
| Mn2+ | requirement | Cucumis sativus | |
| Mn2+ | less effective than Mg2+ | Cucumis sativus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + D-fructose 6-phosphate | Cucumis sativus | - |
phosphate + D-fructose 1,6-bisphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cucumis sativus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| more than 700fold | Cucumis sativus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | germinating | Cucumis sativus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.2 | - |
purified enzyme, in presence of D-fructose 2,6-bisphosphate at 0.001 mM | Cucumis sativus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + D-fructose 6-phosphate | - |
Cucumis sativus | phosphate + D-fructose 1,6-bisphosphate | - |
? | |
| diphosphate + D-fructose 6-phosphate | - |
Cucumis sativus | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 7.8 | forward and reverse reaction | Cucumis sativus |
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