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Literature summary for 2.7.1.90 extracted from
- Kinetic mechanism of pyrophosphate-dependent phosphofructokinase from Propionibacterium freudenreichii (1984), Biochemistry, 23, 4101-4108.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,5-Anhydro-D-mannitol 1,6-bisphosphate | D-fructose 1,6-bisphosphate-analog, kinetics | Propionibacterium freudenreichii |  |
| D-fructose 1,6-bisphosphate | forward reaction, product inhibition, kinetics | Propionibacterium freudenreichii | |
| D-fructose 6-phosphate | reverse reaction, product inhibition, kinetics | Propionibacterium freudenreichii | |
| D-tagatose 6-phosphate | kinetics, dead-end substrate | Propionibacterium freudenreichii | |
| diphosphate | - |
Propionibacterium freudenreichii | |
| magnesium diphosphate | reverse reaction, product inhibition, kinetics | Propionibacterium freudenreichii | |
| Methylenediphosphonate | kinetics, diphosphate analog | Propionibacterium freudenreichii | |
| phosphate | forward reaction, product inhibition, kinetics | Propionibacterium freudenreichii | |
| SO42- | phosphate analog, kinetics | Propionibacterium freudenreichii | |
| Tetrapolyphosphate | - |
Propionibacterium freudenreichii | |
| tripolyphosphate | - |
Propionibacterium freudenreichii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Propionibacterium freudenreichii | |
| 0.005 | - |
diphosphate | as Mg2+ complex, pH 8.0, 25°C | Propionibacterium freudenreichii | |
| 0.03 | - |
D-fructose 1,6-bisphosphate | reverse reaction, pH 8.0, 25°C | Propionibacterium freudenreichii | |
| 0.1 | - |
Mg2+ | reverse reaction, pH 8.0, 25°C | Propionibacterium freudenreichii | |
| 0.13 | - |
D-fructose 6-phosphate | pH 8.0, 25°C | Propionibacterium freudenreichii | |
| 0.56 | - |
phosphate | pH 8.0, 25°C | Propionibacterium freudenreichii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Propionibacterium freudenreichii | |
| Mg2+ | magnesium diphosphate is the actual substrate of forward reaction | Propionibacterium freudenreichii | |
| Mg2+ | product inhibition of reverse reaction, kinetics | Propionibacterium freudenreichii | |
| Mg2+ | mainly required for the forward reaction | Propionibacterium freudenreichii | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + D-fructose 6-phosphate | Propionibacterium freudenreichii | - |
phosphate + D-fructose 1,6-bisphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Propionibacterium freudenreichii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to apparent homogeneity | Propionibacterium freudenreichii |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| diphosphate + D-fructose 6-phosphate = phosphate + D-fructose 1,6-bisphosphate | random terreactant or rapid equilibrium random bi-ter mechanism | Propionibacterium freudenreichii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + D-fructose 6-phosphate | - |
Propionibacterium freudenreichii | phosphate + D-fructose 1,6-bisphosphate | - |
? | |
| diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Propionibacterium freudenreichii | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Propionibacterium freudenreichii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Propionibacterium freudenreichii |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | inhibition kinetics: inhibition by product and inhibitors | Propionibacterium freudenreichii | |
| 0.023 | - |
diphosphate | versus phosphate, pH 8.0, 25°C | Propionibacterium freudenreichii | |
| 0.086 | - |
D-fructose 1,6-bisphosphate | versus D-fructose 6-phosphate, pH 8.0, 25°C | Propionibacterium freudenreichii | |
| 0.62 | - |
D-fructose 6-phosphate | versus D-fructose 1,6-bisphosphate, pH 8.0, 25°C | Propionibacterium freudenreichii | |
| 10.3 | - |
phosphate | versus diphosphate, pH 8.0, 25°C | Propionibacterium freudenreichii | |
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