Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose 2,6-bisphosphate | 60fold increase in affinity for fructose 6-phosphate and 3-5fold for fructose 1,6-bisohosphate | Brassica nigra | |
D-fructose 2,6-bisphosphate | shifts pH-optimum for both reaction directions to slightly acidic | Brassica nigra |
General Stability | Organism |
---|---|
chymostatin and leupeptin stabilize the enzyme and prevent degradation during purification | Brassica nigra |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-phospho-D-glycerate | no inhibition | Brassica nigra | |
ADP | forward reaction, as Mg2+-complex | Brassica nigra | |
AMP | no inhibition | Brassica nigra | |
ATP | forward reaction, as Mg2+-complex | Brassica nigra | |
citrate | no inhibition | Brassica nigra | |
D-glucose 6-phosphate | no inhibition | Brassica nigra | |
diphosphate | reverse reaction | Brassica nigra | |
additional information | no inhibition by 2-phosphoglycerate | Brassica nigra | |
phosphate | forward reaction, product inhibition, kinetics | Brassica nigra | |
phosphoenolpyruvate | forward reaction | Brassica nigra |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | 0.017 | diphosphate | pH 6.8, 25°C, in presence of fructose 2,6-bisphosphate | Brassica nigra | |
0.05 | - |
D-fructose 6-phosphate | pH 6.8, 25°C, in presence of fructose 2,6-bisphosphate | Brassica nigra | |
0.09 | - |
D-fructose 1,6-bisphosphate | pH 6.8, 25°C, in presence of fructose 2,6-bisphosphate | Brassica nigra | |
0.25 | - |
phosphate | pH 6.8, 25°C, in presence of fructose 2,6-bisphosphate | Brassica nigra |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Brassica nigra |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
4 * 60000 + 4 * 66000, SDS-PAGE | Brassica nigra |
66000 | - |
4 * 60000 + 4 * 66000, SDS-PAGE | Brassica nigra |
460000 | - |
gel filtration | Brassica nigra |
520000 | - |
native PAGE | Brassica nigra |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica nigra | - |
- |
- |
Purification (Comment) | Organism |
---|---|
184fold | Brassica nigra |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Brassica nigra | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
26 | - |
purified enzyme | Brassica nigra |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Brassica nigra | phosphate + D-fructose 1,6-bisphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
octamer | 4 * 60000 + 4 * 66000, SDS-PAGE | Brassica nigra |
Synonyms | Comment | Organism |
---|---|---|
PFP | - |
Brassica nigra |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Brassica nigra |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
forward reaction in presence of fructose 2,6-bisphosphate | Brassica nigra |
7.6 | - |
reverse reaction, broad optimum, in both presence and absence of fructose 2,6-bisphosphate | Brassica nigra |
7.6 | - |
forward reaction in absence of fructose 2,6-bisphosphate | Brassica nigra |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7.6 | reverse reaction, 96% activity at pH 6.8, maximum at pH 7.6 | Brassica nigra |