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Literature summary for 2.7.1.90 extracted from
- Physiological relevance of fructose 2,6-bisphosphate in the regulation of spinach leaf pyrophosphate:fructose 6-phosphate 1-phosphotransferase (2001), Planta, 213, 147-157.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose 2,6-bisphosphate | activating effect is greatly influenced by substrates of both directions, phosphoenolpyruvate, 3-phosphoglycerate, and ATP, kinetics | Spinacia oleracea |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-phospho-D-glycerate | diminishes the activating effect of fructose 2,6-bisphosphate | Spinacia oleracea | |
| ATP | diminishes the activating effect of fructose 2,6-bisphosphate | Spinacia oleracea | |
| D-fructose 6-phosphate | reverse reaction, diminishes the activating effect of fructose 2,6-bisphosphate | Spinacia oleracea | |
| diphosphate | reverse reaction, diminishes the activating effect of fructose 2,6-bisphosphate | Spinacia oleracea | |
| phosphate | forward reaction, diminishes the activating effect of fructose 2,6-bisphosphate | Spinacia oleracea | |
| phosphoenolpyruvate | diminishes the activating effect of fructose 2,6-bisphosphate | Spinacia oleracea | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Spinacia oleracea |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Spinacia oleracea | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 1066fold, to near homogeneity | Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 36.3 | - |
purified enzyme | Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Spinacia oleracea | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Spinacia oleracea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Spinacia oleracea |
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Release 2023.1 (January 2023)
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