Activating Compound | Comment | Organism | Structure |
---|---|---|---|
AMP | no effect | Thermoproteus tenax | |
ATP | no effect | Thermoproteus tenax | |
D-fructose 2,6-bisphosphate | no activation | Thermoproteus tenax | |
D-glucose | no effect | Thermoproteus tenax | |
additional information | no effect of ADP, pyruvate, citrate, phosphoenolpyruvate | Thermoproteus tenax |
General Stability | Organism |
---|---|
dialysis in absence of KCl or MgCl2 results in total loss of enzyme activity | Thermoproteus tenax |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | no inhibition | Thermoproteus tenax | |
AMP | no inhibition | Thermoproteus tenax | |
ATP | no inhibition | Thermoproteus tenax | |
citrate | no inhibition | Thermoproteus tenax | |
EDTA | - |
Thermoproteus tenax | |
additional information | no effect of glucose, pyruvate, fructose 2,6-bisphosphate | Thermoproteus tenax | |
phosphoenolpyruvate | no inhibition | Thermoproteus tenax |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
divalent cations | required | Thermoproteus tenax | |
Mg2+ | requirement | Thermoproteus tenax |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
4 * 37000, SDS-PAGE | Thermoproteus tenax |
100000 | - |
gel filtration | Thermoproteus tenax |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoproteus tenax | - |
- |
- |
Purification (Comment) | Organism |
---|---|
74fold, to homogeneity | Thermoproteus tenax |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.5 | - |
purified enzyme, assay temperature 50°C is not the temperature optimum | Thermoproteus tenax |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + D-fructose 6-phosphate | specific for D-fructose 6-phosphate and D-fructose 1,6-bisphosphate | Thermoproteus tenax | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Thermoproteus tenax | phosphate + D-fructose 1,6-bisphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 37000, SDS-PAGE | Thermoproteus tenax |
Synonyms | Comment | Organism |
---|---|---|
PPi-PFK | - |
Thermoproteus tenax |
pyrophosphate-dependent phosphofructokinase | - |
Thermoproteus tenax |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Thermoproteus tenax |
86 | - |
- |
Thermoproteus tenax |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Thermoproteus tenax |