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Literature summary for 2.7.1.91 extracted from

  • Don, A.S.; Martinez-Lamenca, C.; Webb, W.R.; Proia, R.L.; Roberts, E.; Rosen, H.
    Essential requirement for sphingosine kinase 2 in a sphingolipid apoptosis pathway activated by FTY720 analogues (2007), J. Biol. Chem., 282, 15833-15842.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0076
-
sphingosine in 50 mM HEPES, pH 7.4, 10 mM KCl, 15 mM MgCl2, 0.1% Triton X-100, 20% glycerol, 2 mM orthovanadate, 2 mM dithiothreitol, 10 mM NaF, and 1 mM deoxypyridoxine Homo sapiens

Organic Solvent Stability

Organic Solvent Comment Organism
Glycerol stable when stored at -20°C in the presence of 50% glycerol Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9NRA0
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
Homo sapiens
-
JURKAT cell
-
Homo sapiens
-

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol, remains stable Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + sphingosine
-
Homo sapiens ADP + sphingosine 1-phosphate
-
r

Synonyms

Synonyms Comment Organism
sphinganine kinase
-
Homo sapiens
SPHK2
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens