Any feedback?
Literature summary for 2.7.1.95 extracted from
- Aminoglycoside-modifying enzymes (1975), Methods Enzymol., 43, 611-627.
General Stability
| General Stability | Organism |
|---|---|
| ammonium chloride or neomycin B stabilizes, E. coli JR39 enzyme | Escherichia coli |
| dithiothreitol stabilizes and restores activity of denatured enzyme | Staphylococcus aureus |
| dithiothreitol stabilizes and restores activity of denatured enzyme | Escherichia coli |
| dithiothreitol stabilizes and restores activity of denatured enzyme | Pseudomonas aeruginosa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Gentamicin 1a | with neomycin as substrate, no inhibition in E. coli strain JR66/W677 or Pseudomonas aeruginosa strain Ps49 | Escherichia coli |  |
| Gentamicin 1a | with neomycin as substrate, no inhibition in E. coli strain JR66/W677 or Pseudomonas aeruginosa strain Ps49 | Pseudomonas aeruginosa | |
| kanamycin | substrate inhibition at 2 mM | Staphylococcus aureus | |
| tobramycin | - |
Escherichia coli | |
| tobramycin | - |
Pseudomonas aeruginosa | |
| tobramycin | - |
Staphylococcus aureus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
kanamycin | pH 7.5, 35°C | Pseudomonas aeruginosa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activation at 1 mM, can replace Mg2+ | Pseudomonas aeruginosa | |
| Mg2+ | - |
Pseudomonas aeruginosa | |
| Mg2+ | required for activity | Escherichia coli | |
| Mn2+ | activation at 1 mM, can replace Mg2+ | Pseudomonas aeruginosa | |
| Zn2+ | activation at 1 mM, can replace Mg2+ | Pseudomonas aeruginosa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
strains JR35/W677, JR39/W677, JR66/W677, neomycin phosphotransferase II, ML1629, neomycin phosphotransferase I | - |
| Pseudomonas aeruginosa | - |
neomycin phosphotransferase II | - |
| Pseudomonas aeruginosa Ps49 | - |
neomycin phosphotransferase II | - |
| Staphylococcus aureus | - |
multiple drug-resistant strain B294 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + kanamycin | specific for ATP | Staphylococcus aureus | ADP + kanamycin 3'-phosphate | - |
? | |
| ATP + kanamycin | specific for ATP | Escherichia coli | ADP + kanamycin 3'-phosphate | - |
? | |
| ATP + kanamycin | specific for ATP | Pseudomonas aeruginosa | ADP + kanamycin 3'-phosphate | - |
? | |
| ATP + kanamycin | specific for ATP | Pseudomonas aeruginosa Ps49 | ADP + kanamycin 3'-phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 37 | assay at | Staphylococcus aureus |
| 35 | 37 | assay at | Escherichia coli |
| 35 | 37 | assay at | Pseudomonas aeruginosa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
strain ML1629, 5 min, inactivation | Escherichia coli |
| 55 | - |
strain H-9, 20 min stable | Pseudomonas aeruginosa |
| 65 | - |
strain H-9, 5 min stable | Pseudomonas aeruginosa |
| 75 | - |
strain H-9, inactivation | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8 | broad, no marked optimum | Escherichia coli |
| 5.5 | 8 | broad, no marked optimum | Pseudomonas aeruginosa |
| 7 | 7.5 | - |
Staphylococcus aureus |
| 7.5 | - |
with kanamycin | Pseudomonas aeruginosa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9 | stable | Pseudomonas aeruginosa |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
