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Literature summary for 2.7.2.1 extracted from

  • Nakajima, H.; Suzuki, K.; Imahori, K.
    Purification and properties of acetate kinase from Bacillus stearothermophilus (1978), J. Biochem., 84, 193-203.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Geobacillus stearothermophilus

General Stability

General Stability Organism
ATP or ADP protects the enzyme from inactivation Geobacillus stearothermophilus

Inhibitors

Inhibitors Comment Organism Structure
2,4,6-Trinitrobenzene sulfonic acid
-
Geobacillus stearothermophilus
additional information not inactivated by 5,5'-dithiobis(2-nitrobenzoic acid), tetranitromethane or 2-hydroxy-3-nitro-benzyl bromide Geobacillus stearothermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Geobacillus stearothermophilus
Co2+
-
Geobacillus stearothermophilus
Mg2+
-
Geobacillus stearothermophilus
Mn2+
-
Geobacillus stearothermophilus
Ni2+
-
Geobacillus stearothermophilus
Zn2+
-
Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
4 * 43000, SDS-PAGE Geobacillus stearothermophilus
160000
-
gel filtration Geobacillus stearothermophilus
170000
-
sedimentation analysis Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + acetate Geobacillus stearothermophilus
-
ADP + acetyl phosphate
-
r
ATP + acetate Geobacillus stearothermophilus NCA 1503
-
ADP + acetyl phosphate
-
r

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
NCA 1503
-
Geobacillus stearothermophilus NCA 1503
-
NCA 1503
-

Oxidation Stability

Oxidation Stability Organism
sensitive to light oxidation Geobacillus stearothermophilus

Purification (Commentary)

Purification (Comment) Organism
-
Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.71
-
-
Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetate
-
Geobacillus stearothermophilus ADP + acetyl phosphate
-
r
ATP + acetate
-
Geobacillus stearothermophilus NCA 1503 ADP + acetyl phosphate
-
r
ATP + propionate
-
Geobacillus stearothermophilus ADP + propionyl phosphate
-
r
CTP + acetate
-
Geobacillus stearothermophilus CDP + acetyl phosphate
-
r
CTP + acetate
-
Geobacillus stearothermophilus NCA 1503 CDP + acetyl phosphate
-
r
GTP + acetate
-
Geobacillus stearothermophilus GDP + acetyl phosphate
-
r
GTP + acetate
-
Geobacillus stearothermophilus NCA 1503 GDP + acetyl phosphate
-
r
additional information formate is no substrate Geobacillus stearothermophilus ?
-
?
additional information formate is no substrate Geobacillus stearothermophilus NCA 1503 ?
-
?
UTP + acetate
-
Geobacillus stearothermophilus UDP + acetyl phosphate
-
r
UTP + acetate
-
Geobacillus stearothermophilus NCA 1503 UDP + acetyl phosphate
-
r

Subunits

Subunits Comment Organism
tetramer 4 * 43000, SDS-PAGE Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
quite stable up to Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
-
Geobacillus stearothermophilus

pH Range

pH Minimum pH Maximum Comment Organism
6 9.5
-
Geobacillus stearothermophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 11 rather stable at alkaline pH but rapidly inactivated at acidic pH lower than 6.0, about 40% loss of activity at pH 11.0 Geobacillus stearothermophilus