Crystallization (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
General Stability | Organism |
---|---|
ATP or ADP protects the enzyme from inactivation | Geobacillus stearothermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4,6-Trinitrobenzene sulfonic acid | - |
Geobacillus stearothermophilus | |
additional information | not inactivated by 5,5'-dithiobis(2-nitrobenzoic acid), tetranitromethane or 2-hydroxy-3-nitro-benzyl bromide | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Geobacillus stearothermophilus | |
Co2+ | - |
Geobacillus stearothermophilus | |
Mg2+ | - |
Geobacillus stearothermophilus | |
Mn2+ | - |
Geobacillus stearothermophilus | |
Ni2+ | - |
Geobacillus stearothermophilus | |
Zn2+ | - |
Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
4 * 43000, SDS-PAGE | Geobacillus stearothermophilus |
160000 | - |
gel filtration | Geobacillus stearothermophilus |
170000 | - |
sedimentation analysis | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate | Geobacillus stearothermophilus | - |
ADP + acetyl phosphate | - |
r | |
ATP + acetate | Geobacillus stearothermophilus NCA 1503 | - |
ADP + acetyl phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
NCA 1503 | - |
Geobacillus stearothermophilus NCA 1503 | - |
NCA 1503 | - |
Oxidation Stability | Organism |
---|---|
sensitive to light oxidation | Geobacillus stearothermophilus |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.71 | - |
- |
Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate | - |
Geobacillus stearothermophilus | ADP + acetyl phosphate | - |
r | |
ATP + acetate | - |
Geobacillus stearothermophilus NCA 1503 | ADP + acetyl phosphate | - |
r | |
ATP + propionate | - |
Geobacillus stearothermophilus | ADP + propionyl phosphate | - |
r | |
CTP + acetate | - |
Geobacillus stearothermophilus | CDP + acetyl phosphate | - |
r | |
CTP + acetate | - |
Geobacillus stearothermophilus NCA 1503 | CDP + acetyl phosphate | - |
r | |
GTP + acetate | - |
Geobacillus stearothermophilus | GDP + acetyl phosphate | - |
r | |
GTP + acetate | - |
Geobacillus stearothermophilus NCA 1503 | GDP + acetyl phosphate | - |
r | |
additional information | formate is no substrate | Geobacillus stearothermophilus | ? | - |
? | |
additional information | formate is no substrate | Geobacillus stearothermophilus NCA 1503 | ? | - |
? | |
UTP + acetate | - |
Geobacillus stearothermophilus | UDP + acetyl phosphate | - |
r | |
UTP + acetate | - |
Geobacillus stearothermophilus NCA 1503 | UDP + acetyl phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 43000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
quite stable up to | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
- |
Geobacillus stearothermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | - |
Geobacillus stearothermophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | rather stable at alkaline pH but rapidly inactivated at acidic pH lower than 6.0, about 40% loss of activity at pH 11.0 | Geobacillus stearothermophilus |