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Literature summary for 2.7.2.1 extracted from
- Cloning, expression, and characterization of acetate kinase from Lactobacillus sanfranciscensis (2001), Microbiol. Res., 156, 267-277.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| ack gene identified by PCR, heterologously expressed in Escherichia coli JM 109 | Fructilactobacillus sanfranciscensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
ADP | pH 7.5, 30°C | Fructilactobacillus sanfranciscensis |  |
| 0.6 | - |
ATP | pH 7.5, 30°C | Fructilactobacillus sanfranciscensis | |
| 1 | - |
acetyl phosphate | pH 7.5, 30°C | Fructilactobacillus sanfranciscensis | |
| 48 | - |
acetate | pH 7.5, 30°C | Fructilactobacillus sanfranciscensis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Fructilactobacillus sanfranciscensis | |
| Mn2+ | - |
Fructilactobacillus sanfranciscensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
SDS-PAGE | Fructilactobacillus sanfranciscensis |
| 57000 | - |
gel filtration | Fructilactobacillus sanfranciscensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetate | Fructilactobacillus sanfranciscensis | key enzyme and responsible for dephosphorylation of acetyl phosphate with the concomitant production of acetate and ATP | ADP + acetyl phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fructilactobacillus sanfranciscensis | Q9F1X7 | DSM20451, same nucleotide sequence accession number in EMBL/GenBank | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Fructilactobacillus sanfranciscensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
190 U/mg | Fructilactobacillus sanfranciscensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetate | - |
Fructilactobacillus sanfranciscensis | ADP + acetyl phosphate | - |
r | |
| ATP + acetate | key enzyme and responsible for dephosphorylation of acetyl phosphate with the concomitant production of acetate and ATP | Fructilactobacillus sanfranciscensis | ADP + acetyl phosphate | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 53 | - |
- |
Fructilactobacillus sanfranciscensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
80 | - |
Fructilactobacillus sanfranciscensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 90 | stable for 30 min at 60°C, loses activity after 30 min at 70°C, 10 min at 80°C and 5 min at 90°C | Fructilactobacillus sanfranciscensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Fructilactobacillus sanfranciscensis |
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