Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-oxo-L-Pro | 12 mM, 10% inhibition | Pseudomonas aeruginosa | |
ADP | - |
Pseudomonas aeruginosa | |
Cd2+ | 0.1 mM, complete inhibition | Pseudomonas aeruginosa | |
DL-3,4-Didehydroproline | 9 mM, 50% inhibition | Pseudomonas aeruginosa | |
Hg2+ | 0.1 mM, complete inhibition | Pseudomonas aeruginosa | |
iodoacetamide | 0.125 mM, 60% inhibition. Preincubation with 0.25 mM dithiothreitol for 5 min partially protects | Pseudomonas aeruginosa | |
L-azetidine-2-carboxylic acid | 3 mM, 50% inhibition | Pseudomonas aeruginosa | |
L-methionine-DL-sulphoximine | competitive with L-glutamate | Pseudomonas aeruginosa | |
L-Orn | 12 mM, 10% inhibition | Pseudomonas aeruginosa | |
L-Pro | enzyme from strain PAO1: 5 mM, 50% inhibition, complete inhibition at 30 mM, noncompetitive. Strain PAO 879, a proline-auxotroph mutant lacks a proline-inhibitable gamma-glutamyl kinase | Pseudomonas aeruginosa | |
L-Thioproline | 12 mM, 10% inhibition | Pseudomonas aeruginosa | |
Mg2+ | above 20 mM | Pseudomonas aeruginosa | |
Mn2+ | above 20 mM | Pseudomonas aeruginosa | |
NEM | 0.125 mM, complete inhibition. Preincubation with 0.25 mM dithiothreitol for 5 min partially protects | Pseudomonas aeruginosa | |
PCMB | 0.125 mM, complete inhibition. Preincubation with 0.25 mM dithiothreitol for 5 min partially protects | Pseudomonas aeruginosa | |
phosphate | - |
Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12 | - |
L-glutamate | - |
Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Pseudomonas aeruginosa | |
Mn2+ | can partially replace Mg2+ | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
84000 | - |
gel filtration | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Pseudomonas aeruginosa PAO 1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12.68 | - |
- |
Pseudomonas aeruginosa |
Storage Stability | Organism |
---|---|
-40°C, 40% loss of activity within 2 months, then remains stable | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-ethyl-L-glutamate | 5% of the activity with L-glutamate | Pseudomonas aeruginosa | ? | - |
? | |
ATP + 5-ethyl-L-glutamate | 5% of the activity with L-glutamate | Pseudomonas aeruginosa PAO 1 | ? | - |
? | |
ATP + 5-methyl-L-glutamate | 6% of the activity with L-glutamate | Pseudomonas aeruginosa | ? | - |
? | |
ATP + 5-methyl-L-glutamate | 6% of the activity with L-glutamate | Pseudomonas aeruginosa PAO 1 | ? | - |
? | |
ATP + L-glutamate | - |
Pseudomonas aeruginosa | ADP + L-glutamate 5-phosphate | - |
? | |
ATP + L-glutamate | - |
Pseudomonas aeruginosa PAO 1 | ADP + L-glutamate 5-phosphate | - |
? | |
ATP + L-glutamine | 10% of the activity with L-glutamate | Pseudomonas aeruginosa | ? | - |
? | |
ATP + L-glutamine | 10% of the activity with L-glutamate | Pseudomonas aeruginosa PAO 1 | ? | - |
? | |
GTP + L-glutamate | 10% of the activity with ATP | Pseudomonas aeruginosa | GDP + L-glutamate 5-phosphate | - |
? | |
GTP + L-glutamate | 10% of the activity with ATP | Pseudomonas aeruginosa PAO 1 | GDP + L-glutamate 5-phosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.3 | - |
Pseudomonas aeruginosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | pH 5.5: about 80% of maximal activity, pH 8.5: about 40% of maximal activity | Pseudomonas aeruginosa |