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Literature summary for 2.7.2.11 extracted from

  • Krishna, R.V.; Leisinger, T.
    Biosynthesis of proline in Pseudomonas aeruginosa. Partial purification and characterization of gamma-glutamyl kinase (1979), Biochem. J., 181, 215-222.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
5-oxo-L-Pro 12 mM, 10% inhibition Pseudomonas aeruginosa
ADP
-
Pseudomonas aeruginosa
Cd2+ 0.1 mM, complete inhibition Pseudomonas aeruginosa
DL-3,4-Didehydroproline 9 mM, 50% inhibition Pseudomonas aeruginosa
Hg2+ 0.1 mM, complete inhibition Pseudomonas aeruginosa
iodoacetamide 0.125 mM, 60% inhibition. Preincubation with 0.25 mM dithiothreitol for 5 min partially protects Pseudomonas aeruginosa
L-azetidine-2-carboxylic acid 3 mM, 50% inhibition Pseudomonas aeruginosa
L-methionine-DL-sulphoximine competitive with L-glutamate Pseudomonas aeruginosa
L-Orn 12 mM, 10% inhibition Pseudomonas aeruginosa
L-Pro enzyme from strain PAO1: 5 mM, 50% inhibition, complete inhibition at 30 mM, noncompetitive. Strain PAO 879, a proline-auxotroph mutant lacks a proline-inhibitable gamma-glutamyl kinase Pseudomonas aeruginosa
L-Thioproline 12 mM, 10% inhibition Pseudomonas aeruginosa
Mg2+ above 20 mM Pseudomonas aeruginosa
Mn2+ above 20 mM Pseudomonas aeruginosa
NEM 0.125 mM, complete inhibition. Preincubation with 0.25 mM dithiothreitol for 5 min partially protects Pseudomonas aeruginosa
PCMB 0.125 mM, complete inhibition. Preincubation with 0.25 mM dithiothreitol for 5 min partially protects Pseudomonas aeruginosa
phosphate
-
Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
12
-
L-glutamate
-
Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Pseudomonas aeruginosa
Mn2+ can partially replace Mg2+ Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
84000
-
gel filtration Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-
Pseudomonas aeruginosa PAO 1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.68
-
-
Pseudomonas aeruginosa

Storage Stability

Storage Stability Organism
-40°C, 40% loss of activity within 2 months, then remains stable Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5-ethyl-L-glutamate 5% of the activity with L-glutamate Pseudomonas aeruginosa ?
-
?
ATP + 5-ethyl-L-glutamate 5% of the activity with L-glutamate Pseudomonas aeruginosa PAO 1 ?
-
?
ATP + 5-methyl-L-glutamate 6% of the activity with L-glutamate Pseudomonas aeruginosa ?
-
?
ATP + 5-methyl-L-glutamate 6% of the activity with L-glutamate Pseudomonas aeruginosa PAO 1 ?
-
?
ATP + L-glutamate
-
Pseudomonas aeruginosa ADP + L-glutamate 5-phosphate
-
?
ATP + L-glutamate
-
Pseudomonas aeruginosa PAO 1 ADP + L-glutamate 5-phosphate
-
?
ATP + L-glutamine 10% of the activity with L-glutamate Pseudomonas aeruginosa ?
-
?
ATP + L-glutamine 10% of the activity with L-glutamate Pseudomonas aeruginosa PAO 1 ?
-
?
GTP + L-glutamate 10% of the activity with ATP Pseudomonas aeruginosa GDP + L-glutamate 5-phosphate
-
?
GTP + L-glutamate 10% of the activity with ATP Pseudomonas aeruginosa PAO 1 GDP + L-glutamate 5-phosphate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.3
-
Pseudomonas aeruginosa

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.5 pH 5.5: about 80% of maximal activity, pH 8.5: about 40% of maximal activity Pseudomonas aeruginosa