General Stability | Organism |
---|---|
bovine serum albumin and dithioerythritol stabilize in the enzyme assay | Mus musculus |
bovine serum albumin and dithioerythritol stabilize in the enzyme assay | Homo sapiens |
bovine serum albumin and dithioerythritol stabilize in the enzyme assay | Oryctolagus cuniculus |
bovine serum albumin and dithioerythritol stabilize in the enzyme assay | Equus caballus |
bovine serum albumin and dithioerythritol stabilize in the enzyme assay | trout |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3-diphosphoglycerate | competitive to 3-phospho-D-glycerate and MgATP2- | Equus caballus | |
2,3-diphosphoglycerate | competitive to 3-phospho-D-glycerate and MgATP2- | Homo sapiens | |
2,3-diphosphoglycerate | competitive to 3-phospho-D-glycerate and MgATP2- | Mus musculus | |
2,3-diphosphoglycerate | competitive to 3-phospho-D-glycerate and MgATP2- | Oryctolagus cuniculus | |
2,3-diphosphoglycerate | competitive to 3-phospho-D-glycerate and MgATP2- | trout | |
5,5'-dithiobis(2-nitrobenzoic acid) | - |
Equus caballus | |
5,5'-dithiobis(2-nitrobenzoic acid) | - |
Homo sapiens | |
5,5'-dithiobis(2-nitrobenzoic acid) | - |
Mus musculus | |
5,5'-dithiobis(2-nitrobenzoic acid) | - |
Oryctolagus cuniculus | |
5,5'-dithiobis(2-nitrobenzoic acid) | - |
trout | |
AMP | - |
Equus caballus | |
AMP | - |
Homo sapiens | |
AMP | - |
Mus musculus | |
AMP | - |
Oryctolagus cuniculus | |
AMP | - |
trout | |
GMP | - |
Equus caballus | |
GMP | - |
Homo sapiens | |
GMP | - |
Mus musculus | |
GMP | - |
Oryctolagus cuniculus | |
GMP | - |
trout | |
IMP | - |
Equus caballus | |
IMP | - |
Homo sapiens | |
IMP | - |
Mus musculus | |
IMP | - |
Oryctolagus cuniculus | |
IMP | - |
trout |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Mus musculus | |
additional information | - |
additional information | kinetics | Homo sapiens | |
additional information | - |
additional information | kinetics | Oryctolagus cuniculus | |
additional information | - |
additional information | kinetics | Equus caballus | |
additional information | - |
additional information | kinetics | trout |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
cytosol | - |
Homo sapiens | 5829 | - |
cytosol | - |
Oryctolagus cuniculus | 5829 | - |
cytosol | - |
trout | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44520 | - |
amino acid sequence | Equus caballus |
44660 | - |
erythrocytes, amino acid sequence | Homo sapiens |
46000 | - |
1 * 46000, SDS-PAGE | Homo sapiens |
46000 | - |
1 * 46000, SDS-PAGE | Equus caballus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Equus caballus | - |
- |
- |
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Oryctolagus cuniculus | - |
- |
- |
trout | - |
- |
- |
Purification (Comment) | Organism |
---|---|
101fold from skeletal muscle, 3780fold from erythrocytes | Homo sapiens |
135fold | trout |
70fold | Oryctolagus cuniculus |
99fold from muscle, 333fold from testis | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Homo sapiens | - |
muscle | - |
Mus musculus | - |
muscle | - |
Homo sapiens | - |
muscle | - |
Oryctolagus cuniculus | - |
muscle | - |
Equus caballus | - |
muscle | - |
trout | - |
skeletal muscle | - |
Homo sapiens | - |
skeletal muscle | - |
Oryctolagus cuniculus | - |
skeletal muscle | - |
Equus caballus | - |
testis | - |
Mus musculus | - |
testis | - |
Homo sapiens | - |
testis | - |
Oryctolagus cuniculus | - |
testis | - |
Equus caballus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
600 | - |
purified testis enzyme | Mus musculus |
680 | - |
purified erythrocyte enzyme | Homo sapiens |
695 | - |
purified enzyme | Oryctolagus cuniculus |
700 | - |
- |
Oryctolagus cuniculus |
700 | - |
- |
Equus caballus |
700 | - |
purified enzyme | trout |
710 | - |
purified enzyme | Mus musculus |
710 | - |
purified muscle enzyme | Homo sapiens |
Storage Stability | Organism |
---|---|
as a precipitate in 3.3 M ammonium sulfate, stable for years | Mus musculus |
as a precipitate in 3.3 M ammonium sulfate, stable for years | Oryctolagus cuniculus |
as a precipitate in 3.3 M ammonium sulfate, stable for years | trout |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-phospho-D-glycerate | - |
Mus musculus | ADP + 1,3-diphosphoglycerate | - |
r | |
ATP + 3-phospho-D-glycerate | - |
Homo sapiens | ADP + 1,3-diphosphoglycerate | - |
r | |
ATP + 3-phospho-D-glycerate | - |
Oryctolagus cuniculus | ADP + 1,3-diphosphoglycerate | - |
r | |
ATP + 3-phospho-D-glycerate | - |
Equus caballus | ADP + 1,3-diphosphoglycerate | - |
r | |
ATP + 3-phospho-D-glycerate | - |
trout | ADP + 1,3-diphosphoglycerate | - |
r | |
CTP + 3-phospho-D-glycerate | no activity | Mus musculus | CDP + 1,3-diphosphoglycerate | - |
? | |
CTP + 3-phospho-D-glycerate | no activity | Homo sapiens | CDP + 1,3-diphosphoglycerate | - |
? | |
CTP + 3-phospho-D-glycerate | no activity | Oryctolagus cuniculus | CDP + 1,3-diphosphoglycerate | - |
? | |
CTP + 3-phospho-D-glycerate | no activity | trout | CDP + 1,3-diphosphoglycerate | - |
? | |
dATP + 3-phospho-D-glycerate | - |
Mus musculus | dADP + 1,3-diphosphoglycerate | - |
? | |
dATP + 3-phospho-D-glycerate | - |
Equus caballus | dADP + 1,3-diphosphoglycerate | - |
? | |
dGTP + 3-phospho-D-glycerate | - |
Mus musculus | dGDP + 1,3-diphosphoglycerate | - |
? | |
dGTP + 3-phospho-D-glycerate | - |
Equus caballus | dGDP + 1,3-diphosphoglycerate | - |
? | |
GTP + 3-phospho-D-glycerate | - |
Mus musculus | GDP + 1,3-diphosphoglycerate | - |
? | |
GTP + 3-phospho-D-glycerate | - |
Homo sapiens | GDP + 1,3-diphosphoglycerate | - |
? | |
GTP + 3-phospho-D-glycerate | - |
Oryctolagus cuniculus | GDP + 1,3-diphosphoglycerate | - |
? | |
GTP + 3-phospho-D-glycerate | - |
Equus caballus | GDP + 1,3-diphosphoglycerate | - |
? | |
GTP + 3-phospho-D-glycerate | - |
trout | GDP + 1,3-diphosphoglycerate | - |
? | |
ITP + 3-phospho-D-glycerate | - |
Mus musculus | IDP + 1,3-diphosphoglycerate | - |
? | |
ITP + 3-phospho-D-glycerate | - |
Homo sapiens | IDP + 1,3-diphosphoglycerate | - |
? | |
ITP + 3-phospho-D-glycerate | - |
Oryctolagus cuniculus | IDP + 1,3-diphosphoglycerate | - |
? | |
ITP + 3-phospho-D-glycerate | - |
Equus caballus | IDP + 1,3-diphosphoglycerate | - |
? | |
ITP + 3-phospho-D-glycerate | - |
trout | IDP + 1,3-diphosphoglycerate | - |
? | |
additional information | the only analog of 3-phospho-D-glycerate that can replace the substrate is an artificial 3-phospho-D-glycerate in which the phosphate group-O-PO3H2 is replaced by the phosphomethyl group-CH2-PO3H2, i.e. 2-hydroxy-4-phospho-DL-butyric acid | Mus musculus | ? | - |
? | |
additional information | the only analog of 3-phospho-D-glycerate that can replace the substrate is an artificial 3-phospho-D-glycerate in which the phosphate group-O-PO3H2 is replaced by the phosphomethyl group-CH2-PO3H2, i.e. 2-hydroxy-4-phospho-DL-butyric acid | Homo sapiens | ? | - |
? | |
additional information | the only analog of 3-phospho-D-glycerate that can replace the substrate is an artificial 3-phospho-D-glycerate in which the phosphate group-O-PO3H2 is replaced by the phosphomethyl group-CH2-PO3H2, i.e. 2-hydroxy-4-phospho-DL-butyric acid | Oryctolagus cuniculus | ? | - |
? | |
additional information | the only analog of 3-phospho-D-glycerate that can replace the substrate is an artificial 3-phospho-D-glycerate in which the phosphate group-O-PO3H2 is replaced by the phosphomethyl group-CH2-PO3H2, i.e. 2-hydroxy-4-phospho-DL-butyric acid | Equus caballus | ? | - |
? | |
additional information | the only analog of 3-phospho-D-glycerate that can replace the substrate is an artificial 3-phospho-D-glycerate in which the phosphate group-O-PO3H2 is replaced by the phosphomethyl group-CH2-PO3H2, i.e. 2-hydroxy-4-phospho-DL-butyric acid | trout | ? | - |
? | |
additional information | nucleotide substrate specificity | Mus musculus | ? | - |
? | |
additional information | nucleotide substrate specificity | Homo sapiens | ? | - |
? | |
additional information | nucleotide substrate specificity | Oryctolagus cuniculus | ? | - |
? | |
additional information | nucleotide substrate specificity | trout | ? | - |
? | |
additional information | no activity with dTTP | Mus musculus | ? | - |
? | |
additional information | no activity with dTTP | Homo sapiens | ? | - |
? | |
additional information | no activity with dTTP | Oryctolagus cuniculus | ? | - |
? | |
additional information | no activity with dTTP | trout | ? | - |
? | |
UTP + 3-phospho-D-glycerate | - |
Mus musculus | UDP + 1,3-diphosphoglycerate | - |
? | |
UTP + 3-phospho-D-glycerate | - |
Homo sapiens | UDP + 1,3-diphosphoglycerate | - |
? | |
UTP + 3-phospho-D-glycerate | - |
Oryctolagus cuniculus | UDP + 1,3-diphosphoglycerate | - |
? | |
UTP + 3-phospho-D-glycerate | - |
Equus caballus | UDP + 1,3-diphosphoglycerate | - |
? | |
UTP + 3-phospho-D-glycerate | - |
trout | UDP + 1,3-diphosphoglycerate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Mus musculus |
monomer | - |
Oryctolagus cuniculus |
monomer | - |
trout |
monomer | 1 * 46000, SDS-PAGE | Homo sapiens |
monomer | 1 * 46000, SDS-PAGE | Equus caballus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mus musculus |
25 | - |
assay at | Homo sapiens |
25 | - |
assay at | Oryctolagus cuniculus |
25 | - |
assay at | Equus caballus |
25 | - |
assay at | trout |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
trout |
6 | 8.5 | - |
Homo sapiens |
6 | 9.3 | - |
Oryctolagus cuniculus |
6.5 | 8.5 | - |
Mus musculus |
7.6 | - |
assay at | Mus musculus |
7.6 | - |
assay at | Homo sapiens |
7.6 | - |
assay at | Oryctolagus cuniculus |
7.6 | - |
assay at | Equus caballus |
7.6 | - |
assay at | trout |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Mus musculus | |
ADP | - |
Homo sapiens | |
ADP | - |
Oryctolagus cuniculus | |
ADP | - |
Equus caballus | |
ADP | - |
trout | |
ATP | required as phosphate donor | Mus musculus | |
ATP | required as phosphate donor | Homo sapiens | |
ATP | required as phosphate donor | Oryctolagus cuniculus | |
ATP | required as phosphate donor | Equus caballus | |
ATP | required as phosphate donor | trout | |
dATP | - |
Mus musculus | |
dATP | - |
Equus caballus | |
dGTP | - |
Mus musculus | |
dGTP | - |
Equus caballus | |
GTP | - |
Mus musculus | |
GTP | - |
Homo sapiens | |
GTP | - |
Oryctolagus cuniculus | |
GTP | - |
Equus caballus | |
GTP | - |
trout | |
ITP | - |
Mus musculus | |
ITP | - |
Homo sapiens | |
ITP | - |
Oryctolagus cuniculus | |
ITP | - |
Equus caballus | |
ITP | - |
trout | |
UTP | - |
Mus musculus | |
UTP | - |
Homo sapiens | |
UTP | - |
Oryctolagus cuniculus | |
UTP | - |
Equus caballus | |
UTP | - |
trout |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | - |
2,3-diphosphoglycerate | versus MgATP2- | Mus musculus | |
2.3 | - |
2,3-diphosphoglycerate | versus MgATP2- | Homo sapiens | |
2.3 | - |
2,3-diphosphoglycerate | versus MgATP2- | Oryctolagus cuniculus | |
2.3 | - |
2,3-diphosphoglycerate | versus MgATP2- | Equus caballus | |
2.3 | - |
2,3-diphosphoglycerate | versus MgATP2- | trout | |
3.8 | - |
2,3-diphosphoglycerate | versus 3-phospho-D-glycerate | Mus musculus | |
3.8 | - |
2,3-diphosphoglycerate | versus 3-phospho-D-glycerate | Homo sapiens | |
3.8 | - |
2,3-diphosphoglycerate | versus 3-phospho-D-glycerate | Oryctolagus cuniculus | |
3.8 | - |
2,3-diphosphoglycerate | versus 3-phospho-D-glycerate | Equus caballus | |
3.8 | - |
2,3-diphosphoglycerate | versus 3-phospho-D-glycerate | trout |