Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Zymomonas mobilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | temperature-dependence of the kinetics | Zymomonas mobilis | |
additional information | - |
additional information | temperature-dependence of the kinetics | Thermoanaerobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
gel filtration | Thermoanaerobacter sp. |
44000 | - |
gel filtration | Zymomonas mobilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoanaerobacter sp. | - |
- |
- |
Thermoanaerobacter sp. | - |
formerly Thermoanaerobium brockii strain Rt8.G4 | - |
Thermoanaerobacter sp. Rt8.G4 | - |
- |
- |
Zymomonas mobilis | - |
ATCC 29191 | - |
Purification (Comment) | Organism |
---|---|
- |
Thermoanaerobacter sp. |
native from Zymomonas mobilis, and recombinant from Escherichia coli | Zymomonas mobilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-phospho-D-glycerate | - |
Zymomonas mobilis | ADP + 1,3-diphosphoglycerate | - |
r | |
ATP + 3-phospho-D-glycerate | - |
Thermoanaerobacter sp. | ADP + 1,3-diphosphoglycerate | - |
r | |
ATP + 3-phospho-D-glycerate | - |
Thermoanaerobacter sp. Rt8.G4 | ADP + 1,3-diphosphoglycerate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
63 | - |
- |
Zymomonas mobilis |
70 | - |
- |
Thermoanaerobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermal analysis | Zymomonas mobilis |
additional information | - |
thermal analysis | Thermoanaerobacter sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | temperature-dependence of kinetic | Zymomonas mobilis | |
additional information | - |
additional information | temperature-dependence of kinetic | Thermoanaerobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Thermoanaerobacter sp. |
6 | - |
assay at | Zymomonas mobilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Zymomonas mobilis | |
ADP | - |
Thermoanaerobacter sp. | |
ATP | required as phosphate donor | Zymomonas mobilis | |
ATP | required as phosphate donor | Thermoanaerobacter sp. |