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Literature summary for 2.7.2.3 extracted from

  • Thomas, T.M.; Scopes, R.K.
    The effects of temperature on the kinetics and stability of mesophilic and thermophilic 3-phosphoglycerate kinases (1998), Biochem. J., 330, 1087-1095.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Zymomonas mobilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information temperature-dependence of the kinetics Zymomonas mobilis
additional information
-
additional information temperature-dependence of the kinetics Thermoanaerobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
gel filtration Thermoanaerobacter sp.
44000
-
gel filtration Zymomonas mobilis

Organism

Organism UniProt Comment Textmining
Thermoanaerobacter sp.
-
-
-
Thermoanaerobacter sp.
-
formerly Thermoanaerobium brockii strain Rt8.G4
-
Thermoanaerobacter sp. Rt8.G4
-
-
-
Zymomonas mobilis
-
ATCC 29191
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermoanaerobacter sp.
native from Zymomonas mobilis, and recombinant from Escherichia coli Zymomonas mobilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3-phospho-D-glycerate
-
Zymomonas mobilis ADP + 1,3-diphosphoglycerate
-
r
ATP + 3-phospho-D-glycerate
-
Thermoanaerobacter sp. ADP + 1,3-diphosphoglycerate
-
r
ATP + 3-phospho-D-glycerate
-
Thermoanaerobacter sp. Rt8.G4 ADP + 1,3-diphosphoglycerate
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
63
-
-
Zymomonas mobilis
70
-
-
Thermoanaerobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
thermal analysis Zymomonas mobilis
additional information
-
thermal analysis Thermoanaerobacter sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information temperature-dependence of kinetic Zymomonas mobilis
additional information
-
additional information temperature-dependence of kinetic Thermoanaerobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Thermoanaerobacter sp.
6
-
assay at Zymomonas mobilis

Cofactor

Cofactor Comment Organism Structure
ADP
-
Zymomonas mobilis
ADP
-
Thermoanaerobacter sp.
ATP required as phosphate donor Zymomonas mobilis
ATP required as phosphate donor Thermoanaerobacter sp.