Crystallization (Comment) | Organism |
---|---|
enzyme in ternary complex with Mg2+-bound ATP analogue inhibitors Mg-beta,gamma-methylene-adenosine-5'-triphosphate and Mg-beta,gamma-imido-adenosine-5'-triphosphate, and substrate 3-phospho-D-glycerate, hanging drop vapour diffusion method, reservoir solution: 10 mM beta,gamma-methylene-adenosine-5'-triphosphate, 12 mM MgCl2, 10 mM 3-phospho-D-glycerate, 27-28% w/w polyethylene glycol 8000, pH 7.0, 15°C, a few weeks, X-ray structure determination and analysis | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | nucleotide ligands protect, e.g. MgATP2-, MgADP-, Mg-beta,gamma-methylene-adenosine-5'-triphosphate, Mg-beta,gamma-imido-adenosine-5'-triphosphate | Sus scrofa | |
beta,gamma-imido-adenosine-5'-triphosphate | binding structure; i.e. AMP-PNP, an ATP analogue | Sus scrofa | |
beta,gamma-methylene-adenosine-5'-triphosphate | binding structure; i.e. AMP-PCP, an ATP analogue | Sus scrofa | |
iodoacetamide | nucleotide ligands protect, e.g. MgATP2-, MgADP-, Mg-beta,gamma-methylene-adenosine-5'-triphosphate, Mg-beta,gamma-imido-adenosine-5'-triphosphate | Sus scrofa | |
Mg-beta,gamma-imido-adenosine-5'-triphosphate | - |
Sus scrofa | |
Mg-beta,gamma-methylene-adenosine-5'-triphosphate | - |
Sus scrofa | |
MgADP- | - |
Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | Q7SIB7 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-phospho-D-glycerate | - |
Sus scrofa | ADP + 1,3-diphosphoglycerate | - |
r |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Sus scrofa | |
ATP | required as phosphate donor | Sus scrofa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.039 | - |
MgADP- | pH 7.5, 20°C | Sus scrofa | |
0.58 | - |
Mg-beta,gamma-imido-adenosine-5'-triphosphate | pH 7.5, 20°C | Sus scrofa | |
1.21 | - |
Mg-beta,gamma-methylene-adenosine-5'-triphosphate | pH 7.5, 20°C | Sus scrofa |