Protein Variants | Comment | Organism |
---|---|---|
E343A | kcat for reaction with ATP + 3-phosphoglycerate is 58fold lower than wild-type value, kcat for reaction with ADP + 3-phospho-D-glyceroyl phosphate is 31fold lower than wild-type value | Homo sapiens |
K215A | kcat for reaction with ATP + 3-phosphoglycerate is 1735fold lower than wild-type value, kcat for reaction with ADP + 3-phospho-D-glyceroyl phosphate is 3376fold lower than wild-type value | Homo sapiens |
K219A | kcat for reaction with ATP + 3-phosphoglycerate is 925fold lower than wild-type value, kcat for reaction with ADP + 3-phospho-D-glyceroyl phosphate is 502fold lower than wild-type value | Homo sapiens |
N336A | kcat for reaction with ATP + 3-phosphoglycerate is 139fold lower than wild-type value, kcat for reaction with ADP + 3-phospho-D-glyceroyl phosphate is 113fold lower than wild-type value | Homo sapiens |
R38A | kcat for reaction with ATP + 3-phosphoglycerate is 1851fold lower than wild-type value, kcat for reaction with ADP + 3-phospho-D-glyceroyl phosphate is 439fold lower than wild-type value | Homo sapiens |
T375A | kcat for reaction with ATP + 3-phosphoglycerate is 6.7fold lower than wild-type value, kcat for reaction with ADP + 3-phospho-D-glyceroyl phosphate is 3.9fold lower than wild-type value | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
3-phospho-D-glyceroyl phosphate | mutant enzyme E343A | Homo sapiens | |
0.0022 | - |
3-phospho-D-glyceroyl phosphate | mutant enzyme N336A | Homo sapiens | |
0.003 | - |
3-phospho-D-glyceroyl phosphate | mutant enzyme T375A | Homo sapiens | |
0.0038 | - |
3-phospho-D-glyceroyl phosphate | mutant enzyme K125A | Homo sapiens | |
0.005 | - |
3-phospho-D-glyceroyl phosphate | wild-type enzyme | Homo sapiens | |
0.0077 | - |
3-phospho-D-glyceroyl phosphate | mutant enzyme k219A | Homo sapiens | |
0.1 | - |
3-phospho-D-glycerate | wild-type enzyme | Homo sapiens | |
0.1 | - |
ADP | mutant enzyme R38A | Homo sapiens | |
0.11 | - |
ATP | wild-type enzyme | Homo sapiens | |
0.12 | - |
ADP | wild-type enzyme | Homo sapiens | |
0.15 | - |
ADP | mutant enzyme K125A | Homo sapiens | |
0.29 | - |
3-phospho-D-glyceroyl phosphate | mutant enzyme R38A | Homo sapiens | |
0.31 | - |
3-phospho-D-glycerate | mutant enzyme E343A | Homo sapiens | |
0.41 | - |
3-phospho-D-glycerate | mutant enzyme K125A | Homo sapiens | |
0.42 | - |
ADP | mutant enzyme N336A | Homo sapiens | |
0.54 | - |
3-phospho-D-glycerate | mutant enzyme N336A | Homo sapiens | |
0.63 | - |
3-phospho-D-glycerate | mutant enzyme T375A | Homo sapiens | |
0.91 | - |
ADP | mutant enzyme E343A | Homo sapiens | |
0.96 | - |
ATP | mutant enzyme R38A | Homo sapiens | |
1.04 | - |
ATP | mutant enzyme T375A | Homo sapiens | |
1.12 | - |
ADP | mutant enzyme T375A | Homo sapiens | |
1.34 | - |
ATP | mutant enzyme N336A | Homo sapiens | |
1.42 | - |
ADP | mutant enzyme k219A | Homo sapiens | |
1.69 | - |
ATP | mutant enzyme E343A | Homo sapiens | |
1.72 | - |
3-phospho-D-glycerate | mutant enzyme k219A | Homo sapiens | |
2.47 | - |
ATP | mutant enzyme K125A | Homo sapiens | |
4.43 | - |
ATP | mutant enzyme k219A | Homo sapiens | |
4.5 | - |
3-phospho-D-glycerate | mutant enzyme R38A | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P00558 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + 3-phospho-D-glyceroyl phosphate | catalytic residue R38, which also binds the substrate 3-phosphoglycerate, is essential in inducing domain closure. Similarly, residues K219, N336, and E343 which interact with the nucleotide substrates are involved in the process of domain closure. The other catalytic residue, K215, covers a large distance during catalysis but has no direct role in domain closure | Homo sapiens | ATP + 3-phospho-D-glycerate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-phosphoglycerate kinase | - |
Homo sapiens |
hPGK | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
ATP | mutant enzyme R38A | Homo sapiens | |
0.45 | - |
3-phospho-D-glycerate | mutant enzyme R38A | Homo sapiens | |
0.48 | - |
ATP | mutant enzyme K125A | Homo sapiens | |
0.48 | - |
3-phospho-D-glycerate | mutant enzyme K125A | Homo sapiens | |
0.78 | - |
ADP | mutant enzyme K125A | Homo sapiens | |
0.78 | - |
3-phospho-D-glyceroyl phosphate | mutant enzyme K125A | Homo sapiens | |
0.9 | - |
ATP | mutant enzyme k219A | Homo sapiens | |
0.9 | - |
3-phospho-D-glycerate | mutant enzyme k219A | Homo sapiens | |
5.25 | - |
ADP | mutant enzyme k219A | Homo sapiens | |
5.25 | - |
3-phospho-D-glyceroyl phosphate | mutant enzyme k219A | Homo sapiens | |
6 | - |
ATP | mutant enzyme N336A | Homo sapiens | |
6 | - |
3-phospho-D-glycerate | mutant enzyme N336A | Homo sapiens | |
6 | - |
ADP | mutant enzyme R38A | Homo sapiens | |
6 | - |
3-phospho-D-glyceroyl phosphate | mutant enzyme R38A | Homo sapiens | |
14.3 | - |
ATP | mutant enzyme E343A | Homo sapiens | |
14.3 | - |
3-phospho-D-glycerate | mutant enzyme E343A | Homo sapiens | |
23.2 | - |
3-phospho-D-glyceroyl phosphate | mutant enzyme N336A | Homo sapiens | |
23.3 | - |
ADP | mutant enzyme N336A | Homo sapiens | |
84 | - |
ADP | mutant enzyme E343A | Homo sapiens | |
84 | - |
3-phospho-D-glyceroyl phosphate | mutant enzyme E343A | Homo sapiens | |
125 | - |
ATP | mutant enzyme T375A | Homo sapiens | |
125 | - |
3-phospho-D-glycerate | mutant enzyme T375A | Homo sapiens | |
675 | - |
ADP | mutant enzyme T375A | Homo sapiens | |
675 | - |
3-phospho-D-glyceroyl phosphate | mutant enzyme T375A | Homo sapiens | |
833 | - |
ATP | wild-type enzyme | Homo sapiens | |
833 | - |
3-phospho-D-glycerate | wild-type enzyme | Homo sapiens | |
2633 | - |
ADP | wild-type enzyme | Homo sapiens | |
2633 | - |
3-phospho-D-glyceroyl phosphate | wild-type enzyme | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
diphosphate | mutant enzyme N336A | Homo sapiens | |
0.13 | - |
diphosphate | mutant enzyme R38A | Homo sapiens | |
0.14 | - |
diphosphate | mutant enzyme E343A | Homo sapiens | |
0.22 | - |
diphosphate | wild-type enzyme | Homo sapiens | |
0.51 | - |
diphosphate | mutant enzyme K125A | Homo sapiens | |
0.52 | - |
diphosphate | mutant enzyme k219A | Homo sapiens | |
1.9 | - |
diphosphate | mutant enzyme T375A | Homo sapiens |