Cloned (Comment) | Organism |
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the gene encoding phosphoglycerate kinase is inserted into a yeast expression vector under the control of the galactose-inducible GAL1 yeast promoter. This vector is then transformed into a pgk::TRP1 yeast mutant, a strain inhibited for growth on galactose or glucose due to its lack of PGK enzyme. Slow-growing transformants are obtained on galactose plates at 37°C, but not at 28°C. These transformants contain low levels of transcripts of the heterologous gene and low amounts of thermostable PGK activity. Weak expression of the hyperthermophile gene in yeast, a mesophile, therefore enables complementation of the yeast pgk defect at 37°C but not at 28°C | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
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Saccharolobus solfataricus | - |
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