Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Helianthus annuus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | 0.1 mM, 63.1% activity; 0.1 mM, 85% activity; 0.1 mM, 86% activity; 1 mM, 31% activity; 1 mM, 32% activity | Helianthus annuus | |
AMP | 0.625 mM, 95% activity; 0.625 mM, 98% activity; 10 mM, 40% activity; 10 mM, 44% activity; 10 mM, 50% activity | Helianthus annuus | |
Glycerol 2-phosphate | 10 mM, 97% activity | Helianthus annuus | |
glycerol 3-phosphate | 10 mM, 92% activity; 10 mM, 95% activity; 10 mM, 97% activity | Helianthus annuus | |
UDP | 10 mM, 94.3% activity; 10 mM, 97% activity | Helianthus annuus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.215 | - |
ATP | pH 8.2, 25°C | Helianthus annuus | |
0.227 | - |
ATP | pH 7.6, 25°C | Helianthus annuus | |
0.243 | - |
ATP | pH 7, 25°C | Helianthus annuus | |
0.3 | - |
ATP | pH 7, 25°C | Helianthus annuus | |
0.356 | - |
ATP | pH 8.2, 25°C | Helianthus annuus | |
0.357 | - |
ATP | pH 7, 25°C | Helianthus annuus | |
0.367 | - |
ATP | pH 7.6, 25°C | Helianthus annuus | |
0.404 | - |
ATP | pH 8.2, 25°C | Helianthus annuus | |
0.406 | - |
ATP | pH 7.6, 25°C | Helianthus annuus | |
0.589 | - |
3-phospho-D-glycerate | pH 8.2, 25°C | Helianthus annuus | |
0.601 | - |
3-phospho-D-glycerate | pH 7, 25°C | Helianthus annuus | |
0.683 | - |
3-phospho-D-glycerate | pH 8.2, 25°C | Helianthus annuus | |
0.707 | - |
3-phospho-D-glycerate | pH 7, 25°C | Helianthus annuus | |
0.711 | - |
3-phospho-D-glycerate | pH 7.6, 25°C | Helianthus annuus | |
0.719 | - |
3-phospho-D-glycerate | pH 7.6, 25°C | Helianthus annuus | |
1.635 | - |
3-phospho-D-glycerate | pH 8.2, 25°C | Helianthus annuus | |
1.787 | - |
3-phospho-D-glycerate | pH 7.6, 25°C | Helianthus annuus | |
1.948 | - |
3-phospho-D-glycerate | pH 7, 25°C | Helianthus annuus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Helianthus annuus | 5829 | - |
plastid | - |
Helianthus annuus | 9536 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42300 | - |
calculated from cDNA | Helianthus annuus |
42400 | - |
calculated from cDNA | Helianthus annuus |
45000 | - |
SDS-PAGE, recombinant protein | Helianthus annuus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helianthus annuus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Helianthus annuus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | - |
Helianthus annuus | - |
cotyledon | high expression | Helianthus annuus | - |
leaf | - |
Helianthus annuus | - |
leaf | high expression | Helianthus annuus | - |
root | - |
Helianthus annuus | - |
stem | - |
Helianthus annuus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-phospho-D-glycerate | - |
Helianthus annuus | ADP + 3-phospho-D-glyceroyl phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HacPGK1 | - |
Helianthus annuus |
HacPGK2 | - |
Helianthus annuus |
HapPGK | - |
Helianthus annuus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Helianthus annuus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
80% activity at 65°C, then rapidly decreasing between 65-80°C | Helianthus annuus |
65 | - |
enzymatic activity is completely lost at temperatures over 65°C | Helianthus annuus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Helianthus annuus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.75 | optimal pH range | Helianthus annuus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 9.2 | 75% activity retained at pH 9.2, a sharp reduction of activity below pH 7 | Helianthus annuus |
9.2 | - |
75% activity retained at pH 9.2 | Helianthus annuus |
Organism | Comment | Expression |
---|---|---|
Helianthus annuus | HacPGK1 shows the highest level of expression during seed development, especially at the initial stages, 12 days after flowering (DAF), and accounts for most (approximately 89%) of the combined expression of the three genes. As seed development progresses, the level of expression for this gene decreases | up |
Helianthus annuus | highest expression of HapPGK in photosynthetically active tissues, cotyledons and leaves | up |