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Literature summary for 2.7.2.3 extracted from
- Plastidic phosphoglycerate kinase from Phaeodactylum tricornutum: on the critical role of cysteine residues for the enzyme function (2012), Protist, 163, 188-203.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as a His-tagged fusion protein | Phaeodactylum tricornutum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C214S | similar Vmax as wild-type, mutant behaves in a similar way as wild-type towards oxidants | Phaeodactylum tricornutum |
| C21S | similar Vmax as wild-type, mutant displays slightly lower affinity toward 3-phospho-D-glycerate, mutant behaves in a similar way as wild-type towards oxidants | Phaeodactylum tricornutum |
| C312S | similar Vmax as wild-type, mutant behaves in a similar way as wild-type towards oxidants | Phaeodactylum tricornutum |
| C58S | similar Vmax as wild-type, mutant displays slightly lower affinity toward 3-phospho-D-glycerate, mutant is notably more sensitive to diamide oxidation than wild-type | Phaeodactylum tricornutum |
| C77S | similar Vmax as wild-type, mutant displays slightly lower affinity toward 3-phospho-D-glycerate, mutant is notably more sensitive to diamide oxidation than wild-type | Phaeodactylum tricornutum |
| C95S | similar Vmax as wild-type, mutant displays slightly lower affinity toward 3-phospho-D-glycerate, mutant is notably more sensitive to diamide oxidation than wild-type | Phaeodactylum tricornutum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Diamide | - |
Phaeodactylum tricornutum |  |
| glutathione | - |
Phaeodactylum tricornutum | |
| hydrogen peroxide | - |
Phaeodactylum tricornutum | |
| sodium nitroprusside | - |
Phaeodactylum tricornutum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
gel filtration | Phaeodactylum tricornutum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phaeodactylum tricornutum | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| loses its activity when stored in the absence of of a reducing agent | Phaeodactylum tricornutum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-NTA chromatography | Phaeodactylum tricornutum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 47.5 | - |
pH 8, 30°C, purified enzyme | Phaeodactylum tricornutum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-phospho-D-glycerate | - |
Phaeodactylum tricornutum | ADP + 3-phospho-D-glyceroyl phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | gel filtration, 1 * 45000 | Phaeodactylum tricornutum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PGKase-1 | - |
Phaeodactylum tricornutum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Phaeodactylum tricornutum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Phaeodactylum tricornutum |
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Release 2023.1 (January 2023)
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