Cloned (Comment) | Organism |
---|---|
the gene is cloned into the NheI and BamHI restriction enzyme sites of the expression vector pET28 to create pET28+AKSc, expression in Escherichia coli | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
E254A | 9.1fold decrease in kcat for aspartate and 11fold decrease in kcat for ATP | Saccharomyces cerevisiae |
E279A | kcat is decreased 47 times for aspartate and 44 times for ATP | Saccharomyces cerevisiae |
H292A | 4.5 times increase in Km for ATP and 120 times decrease in kcat for ATP | Saccharomyces cerevisiae |
H292Q | no significant differences to wild type | Saccharomyces cerevisiae |
H497A | kcat is decreased 6.7fold for both substrates | Saccharomyces cerevisiae |
K18A | Km values for both substrates similar to wild type | Saccharomyces cerevisiae |
K18Q | Km values for both substrates similar to wild type | Saccharomyces cerevisiae |
K18R | Km values for aspartate similar to wildtype, Km value for ATP 2.9fold decreased | Saccharomyces cerevisiae |
R419A | 10fold decrease in kcat for aspartate and 8.9fold decrease in kcat for ATP | Saccharomyces cerevisiae |
S23A | differs significantly only in the kcat/Km ratio, which is decreased 4fold for aspartate and 3.7fold for ATP | Saccharomyces cerevisiae |
T22A | Km for ATP increases 4.2fold | Saccharomyces cerevisiae |
T295V | 6.7 times decrease in the kcat/Km ratio for ATP | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-threonine | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
ATP | mutant K18R | Saccharomyces cerevisiae | |
0.61 | - |
ATP | mutant K18Q | Saccharomyces cerevisiae | |
0.7 | - |
ATP | mutant E254A | Saccharomyces cerevisiae | |
0.74 | - |
ATP | wild type | Saccharomyces cerevisiae | |
0.89 | - |
ATP | mutant K18A | Saccharomyces cerevisiae | |
1.05 | - |
ATP | mutant S23A | Saccharomyces cerevisiae | |
1.15 | - |
ATP | mutant E279A | Saccharomyces cerevisiae | |
1.26 | - |
ATP | mutant H497A | Saccharomyces cerevisiae | |
1.45 | - |
ATP | mutant H292Q | Saccharomyces cerevisiae | |
1.63 | - |
L-aspartate | mutant K18R | Saccharomyces cerevisiae | |
2.33 | - |
L-aspartate | mutant E279A | Saccharomyces cerevisiae | |
2.35 | - |
ATP | mutant T295V | Saccharomyces cerevisiae | |
2.4 | - |
ATP | mutant R419A | Saccharomyces cerevisiae | |
3.1 | - |
ATP | mutant T22A | Saccharomyces cerevisiae | |
3.19 | - |
L-aspartate | mutant T295V | Saccharomyces cerevisiae | |
3.21 | - |
L-aspartate | mutant K18A | Saccharomyces cerevisiae | |
3.25 | - |
L-aspartate | mutant R419A | Saccharomyces cerevisiae | |
3.36 | - |
ATP | mutant H292A | Saccharomyces cerevisiae | |
4.25 | - |
L-aspartate | mutant K18Q | Saccharomyces cerevisiae | |
4.59 | - |
L-aspartate | wild type | Saccharomyces cerevisiae | |
6.81 | - |
L-aspartate | mutant H292A | Saccharomyces cerevisiae | |
6.99 | - |
L-aspartate | mutant S23A | Saccharomyces cerevisiae | |
7.08 | - |
L-aspartate | mutant T22A | Saccharomyces cerevisiae | |
8.66 | - |
L-aspartate | mutant H497A | Saccharomyces cerevisiae | |
9.77 | - |
L-aspartate | mutant E254A | Saccharomyces cerevisiae | |
11.9 | - |
L-aspartate | mutant H292Q | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate | Saccharomyces cerevisiae | - |
ADP + phospho-L-aspartate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from E. coli | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate | - |
Saccharomyces cerevisiae | ADP + phospho-L-aspartate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AK | - |
Saccharomyces cerevisiae |
aspartate kinase | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
L-aspartate | mutant K18R | Saccharomyces cerevisiae | |
0.31 | - |
ATP | mutant K18R | Saccharomyces cerevisiae | |
0.41 | - |
ATP | mutant H292A | Saccharomyces cerevisiae | |
0.41 | - |
L-aspartate | mutant K18A | Saccharomyces cerevisiae | |
0.53 | - |
ATP | mutant K18A | Saccharomyces cerevisiae | |
0.54 | - |
L-aspartate | mutant K18Q | Saccharomyces cerevisiae | |
0.6 | - |
ATP | mutant K18Q | Saccharomyces cerevisiae | |
0.98 | - |
L-aspartate | mutant E279A | Saccharomyces cerevisiae | |
1.13 | - |
ATP | mutant E279A | Saccharomyces cerevisiae | |
2.49 | - |
L-aspartate | mutant H292A | Saccharomyces cerevisiae | |
4.43 | - |
ATP | mutant E254A | Saccharomyces cerevisiae | |
4.65 | - |
L-aspartate | mutant R419A | Saccharomyces cerevisiae | |
5.14 | - |
L-aspartate | mutant E254A | Saccharomyces cerevisiae | |
5.55 | - |
ATP | mutant R419A | Saccharomyces cerevisiae | |
6.92 | - |
L-aspartate | mutant H497A | Saccharomyces cerevisiae | |
7.33 | - |
ATP | mutant H497A | Saccharomyces cerevisiae | |
8.3 | - |
ATP | mutant H292Q | Saccharomyces cerevisiae | |
10.3 | - |
L-aspartate | mutant H292Q | Saccharomyces cerevisiae | |
17.9 | - |
L-aspartate | mutant S23A | Saccharomyces cerevisiae | |
18 | - |
L-aspartate | mutant T295V | Saccharomyces cerevisiae | |
19.1 | - |
ATP | mutant S23A | Saccharomyces cerevisiae | |
23.3 | - |
ATP | mutant T295V | Saccharomyces cerevisiae | |
45 | - |
L-aspartate | mutant T22A | Saccharomyces cerevisiae | |
46.6 | - |
L-aspartate | wild type | Saccharomyces cerevisiae | |
49.3 | - |
ATP | wild type | Saccharomyces cerevisiae | |
56.9 | - |
ATP | mutant T22A | Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
L-threonine | wild type, with aspartate as substrate | Saccharomyces cerevisiae | |
3.4 | - |
L-threonine | wild type, with ATP as substrate | Saccharomyces cerevisiae | |
14 | - |
L-threonine | mutant E279A, with aspartate as substrate | Saccharomyces cerevisiae | |
28 | - |
L-threonine | mutant E279A, with ATP as substrate | Saccharomyces cerevisiae |