Literature summary for 2.7.2.4 extracted from

Marina, P.; Martinez-Costa, O.H.; Calderon, I.L.; Aragon, J.J.
Characterization of the aspartate kinase from Saccharomyces cerevisiae and of its interaction with threonine (2004), Biochem. Biophys. Res. Commun., 321, 584-591.

Search for more literature for 2.7.2.4

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli and as fusion protein with GST (glutathione-S-transferase) in Saccharomyces cerevisiae	Saccharomyces cerevisiae

Inhibitors

Inhibitors	Comment	Organism	Structure
L-threonine	allosteric inhibition, causes a decrease in the apparent molecular mass of the enzyme, no inhibition is detected in the threonine insensitive mutant up to 50 mM threonine	Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.8	-	ATP	wild type, in the presence of 10 mM aspartate	Saccharomyces cerevisiae
1.1	-	L-aspartate	threonine insensitive mutant, in the presence of 10 mM ATP	Saccharomyces cerevisiae
1.1	-	L-aspartate	wildtype, in the presence of 10 mM ATP	Saccharomyces cerevisiae
1.2	-	ATP	glutathione-S-transferase fusion protein, in the presence of 10 mM aspartate	Saccharomyces cerevisiae
1.2	-	L-aspartate	glutathione-S-transferase fusion protein, in the presence of 10 mM ATP	Saccharomyces cerevisiae
1.2	-	ATP	threonine insensitive mutant, in the presence of 10 mM aspartate	Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
58000	-	wild type, gel filtration	Saccharomyces cerevisiae
58100	-	wild type, calculated from protein sequence	Saccharomyces cerevisiae
58700	-	wild type, SDS-PAGE	Saccharomyces cerevisiae
280000	-	glutathione-S-transferase fusion protein, in the presence of threonine, gel filtration	Saccharomyces cerevisiae
297000	-	glutathione-S-transferase fusion protein, in the presence of threonine, blue native gel electrophoresis	Saccharomyces cerevisiae
298000	-	wild type, in the presence of threonine, gel filtration	Saccharomyces cerevisiae
344000	-	glutathione-S-transferase fusion protein, gel filtration	Saccharomyces cerevisiae
344000	-	threonine insensitive mutant, with and without threonine in the buffer, blue native gel electrophoresis	Saccharomyces cerevisiae
345000	-	threonine insensitive mutant, with and without threonine in the buffer, gel filtration	Saccharomyces cerevisiae
345000	-	wild type, blue native gel electrophoresis, gel filtration	Saccharomyces cerevisiae
346000	-	wild type, gel filtration	Saccharomyces cerevisiae
346000	-	glutathione-S-transferase fusion protein, blue native gel electrophoresis	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-aspartate	Saccharomyces cerevisiae	-	ADP + phospho-L-aspartate	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
39.05	-	activity after the final purification step	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-aspartate	-	Saccharomyces cerevisiae	ADP + phospho-L-aspartate	-	?

Subunits

Subunits	Comment	Organism
hexamer	6 * 58000, gel filtration, native gel electrophoresis	Saccharomyces cerevisiae
pentamer	glutathione-S-transferase fusion protein, in the presence of threonine, gel filtration	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
AK	-	Saccharomyces cerevisiae
aspartate kinase	-	Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
227	-	L-aspartate	-	Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
1.4	-	L-threonine	glutathione-S-transferase fusion protein, in the presence of 10 mM aspartate and 10 mM ATP	Saccharomyces cerevisiae
1.4	-	L-threonine	wildtype, in the presence of 10 mM aspartate and 10 mM ATP, binds threonine in a hyperbolic manner	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)