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Literature summary for 2.7.2.4 extracted from

  • Yoshida, A.; Tomita, T.; Kuzuyama, T.; Nishiyama, M.
    Mechanism of concerted inhibition of alpha2beta2-type hetero-oligomeric aspartate kinase from Corynebacterium glutamicum (2010), J. Biol. Chem., 285, 27477-27486.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.2.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Corynebacterium glutamicum

Protein Variants

Protein Variants Comment Organism
D45A the inhibition of CgAK by lysine is substantially reduced in D45A mutant Corynebacterium glutamicum
S301F the S301F mutant exhibits resistance to feedback inhibition by lysine and threonine, showing activity in the presence of both lysine and threonine Corynebacterium glutamicum

Inhibitors

Inhibitors Comment Organism Structure
L-lysine enzyme is inhibited by lysine and threonine in a concerted manner: comparison of the crystal structures between inhibitory and active forms reveal that binding inhibitors causes a conformational change to a closed inhibitory form, and the interaction between the catalytic domain in the alpha subunit and beta subunit Corynebacterium glutamicum
L-threonine enzyme is inhibited by lysine and threonine in a concerted manner: comparison of the crystal structures between inhibitory and active forms reveal that binding inhibitors causes a conformational change to a closed inhibitory form, and the interaction between the catalytic domain in the alpha subunit and beta subunit Corynebacterium glutamicum

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate
-
 Corynebacterium glutamicum ADP + 4-phospho-L-aspartate
-
 ?

Subunits

Subunits Comment Organism
heterotetramer crystal structure, heterotetramer composed of two alpha subunits and two beta subunits Corynebacterium glutamicum

Synonyms

Synonyms Comment Organism
CgAK
-
 Corynebacterium glutamicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Corynebacterium glutamicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Corynebacterium glutamicum