General Stability | Organism |
---|---|
L-arginine protects against the inactivating effects of heat | Chlamydomonas reinhardtii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arginine | allosteric enzyme which is inhibited by arginine | Chlamydomonas reinhardtii | |
arginine | enzyme has no allosteric properties and its activity is influenced neither by arginine nor by any of the intermediates of the arginine biosynthetic pathway | Escherichia coli | |
MgCl2 | inhibition above 20 mM | Chlamydomonas reinhardtii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
ATP | - |
Escherichia coli | |
3 | - |
ATP | pH 8.0, 37°C, allosteric | Chlamydomonas reinhardtii | |
4 | - |
N-acetyl-L-glutamate | pH 8.0, 37°C, allosteric | Chlamydomonas reinhardtii | |
6 | - |
N-acetyl-L-glutamate | pH 5.5, 37°C | Escherichia coli | |
15 | - |
N-acetyl-L-glutamate | - |
Chlamydomonas reinhardtii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | Mg2+ or Co2+ required | Chlamydomonas reinhardtii | |
Mg2+ | - |
Escherichia coli | |
Mg2+ | required | Chlamydomonas reinhardtii | |
Mg2+ | Mg2+ or Co2+ required | Chlamydomonas reinhardtii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
400000 | - |
gel filtration | Chlamydomonas reinhardtii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acetyl-L-glutamate | Chlamydomonas reinhardtii | key enzyme in regulation of arginine biosynthesis | ADP + N-acetyl-L-glutamate 5-phosphate | - |
? | |
ATP + N-acetyl-L-glutamate | Escherichia coli | key enzyme in regulation of arginine biosynthesis | ADP + N-acetyl-L-glutamate 5-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | - |
- |
- |
Escherichia coli | - |
ATCC 25542 | - |
Purification (Comment) | Organism |
---|---|
- |
Chlamydomonas reinhardtii |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acetyl-L-glutamate | highly specific for ATP and N-acetyl-L-glutamate | Chlamydomonas reinhardtii | ADP + N-acetyl-L-glutamate 5-phosphate | in presence of hydroxylamine formation of N-acetyl-L-glutamate 5-hydroxamate + ADP + phosphate | ? | |
ATP + N-acetyl-L-glutamate | highly specific for ATP and N-acetyl-L-glutamate | Escherichia coli | ADP + N-acetyl-L-glutamate 5-phosphate | in presence of hydroxylamine formation of N-acetyl-L-glutamate 5-hydroxamate + ADP + phosphate | ? | |
ATP + N-acetyl-L-glutamate | key enzyme in regulation of arginine biosynthesis | Chlamydomonas reinhardtii | ADP + N-acetyl-L-glutamate 5-phosphate | - |
? | |
ATP + N-acetyl-L-glutamate | key enzyme in regulation of arginine biosynthesis | Escherichia coli | ADP + N-acetyl-L-glutamate 5-phosphate | - |
? | |
additional information | not: GTP | Chlamydomonas reinhardtii | ? | - |
? | |
additional information | not: GTP | Escherichia coli | ? | - |
? | |
additional information | not: N-benzoyl-L-glutamate, L-glutamate, D-glutamate | Chlamydomonas reinhardtii | ? | - |
? | |
additional information | not: N-benzoyl-L-glutamate, L-glutamate, D-glutamate | Escherichia coli | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Chlamydomonas reinhardtii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Chlamydomonas reinhardtii |
6.8 | 7.8 | - |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | - |
Chlamydomonas reinhardtii |