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Literature summary for 2.7.2.8 extracted from

  • Maheswaran, M.; Urbanke, C.; Forchhammer, K.
    Complex formation and catalytic activation by the PII signaling protein of N-acetyl-L-glutamate kinase from Synechococcus elongatus strain PCC 7942 (2004), J. Biol. Chem., 279, 55202-55210.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ATP maximum activity at 10 mM Synechococcus elongatus

Inhibitors

Inhibitors Comment Organism Structure
ADP inhibitor of complex formation with PII protein Synechococcus elongatus
arginine feedback inhibition Synechococcus elongatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information formation of complex between enzyme and signal transduction protein PII decreases Km-value by a factor of 10 and increases Vmax 4fold Synechococcus elongatus
2.7
-
N-acetyl-L-glutamate pH 7.5, complex of enzyme and PII protein Synechococcus elongatus
27.4
-
N-acetyl-L-glutamate pH 7.5, free enzyme Synechococcus elongatus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, maximum activity above 20 mM Synechococcus elongatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
6 * 32000, calculated Synechococcus elongatus
180000
-
sedimentation anaylsis Synechococcus elongatus

Organism

Organism UniProt Comment Textmining
Synechococcus elongatus
-
strain PCC 7942, recombinant protein with His6-tag
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.3
-
pH 7.5, free enzyme Synechococcus elongatus
13.3
-
pH 7.5, complex of enzyme and PII protein Synechococcus elongatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + N-acetyl-L-glutamate
-
Synechococcus elongatus ADP + N-acetyl-L-glutamate 5-phosphate
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 32000, calculated Synechococcus elongatus
More enzyme hexamer forms a complex with PII signaling protein trimer Synechococcus elongatus
More in vivo complex of enzyme with signal transduction protein PII Synechococcus elongatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
both free enzyme and complex with protein PII Synechococcus elongatus