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Literature summary for 2.7.3.1 extracted from

  • Shirokane, Y.; Nakajima, M.; Mizusawa, K.
    Purification and properties of guanidinoacetate kinase from a polychaete, Perineis sp. (1991), Agric. Biol. Chem., 55, 2235-2242.
No PubMed abstract available
Search for more literature for 2.7.3.1

Inhibitors

Inhibitors Comment Organism Structure
3-guanidinopropionate slight Perinereis sp.
4-Guanidinobutyrate slight Perinereis sp.
5,5'-dithiobis(2-nitrobenzoate)
-
 Perinereis sp.
agmatine
-
 Perinereis sp.
AMP
-
 Perinereis sp.
Ca2+
-
 Perinereis sp.
Creatine slight Perinereis sp.
guanidine slight Perinereis sp.
Hg2+
-
 Perinereis sp.
iodoacetamide
-
 Perinereis sp.
L-arginine slight Perinereis sp.
methylguanidine slight Perinereis sp.
N-bromosuccinimide
-
 Perinereis sp.
p-chloromercuribenzoate
-
 Perinereis sp.
Taurocyamine slight Perinereis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
 ATP pH 8.1, 25°C Perinereis sp.
4.1
-
 Guanidinoacetate pH 8.1, 25°C Perinereis sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 29% of Mg2+-activation Perinereis sp.
Mg2+ 5 mM, activation Perinereis sp.
Mn2+ 5 mM, activation Perinereis sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 1 * 47000 + 1 * 45000, SDS-PAGE Perinereis sp.
47000
-
 1 * 47000 + 1 * 45000, SDS-PAGE Perinereis sp.
90000
-
 gel filtration Perinereis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + guanidinoacetate Perinereis sp.
-
 ADP + phosphoguanidinoacetate
-
 ?

Organism

Organism UniProt Comment Textmining
Perinereis sp.
-
 polychaete
-

Purification (Commentary)

Purification (Comment) Organism
-
 Perinereis sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
45.3
-
 pH 8.1, 25°C Perinereis sp.

Storage Stability

Storage Stability Organism
-20°C, 20 mM Tris-acetate buffer, pH 8.0, 1 mM dithiothreitol, 5% glycerol, several months Perinereis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + guanidinoacetate
-
 Perinereis sp. ADP + phosphoguanidinoacetate
-
 ?
ATP + guanidinoacetate specific for guanidinoacetate, ITP 2%, GTP 0.5% of ATP activity Perinereis sp. ADP + phosphoguanidinoacetate
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 47000 + 1 * 45000, SDS-PAGE Perinereis sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 phosphorylation of guanidinoacetate Perinereis sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 stable up to Perinereis sp.
40
-
 20 min, 4% loss of activity Perinereis sp.
45
-
 20 min, 28% loss of activity Perinereis sp.
50
-
 inactivation Perinereis sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.1
-
 phosphorylation of guanidinoacetate Perinereis sp.

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9 less than 50% of maximal activity above and below Perinereis sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 9.5
-
 Perinereis sp.