Literature summary for 2.7.3.2 extracted from

Tang, H.M.; Ou, W.B.; Zhou, H.M.
Effects of lactic acid and NaCl on creatine kinase from rabbit muscle (2003), Biochem. Cell Biol., 81, 1-7.

Search for more literature for 2.7.3.2

Inhibitors

Inhibitors	Comment	Organism	Structure
Lactic acid	induces dissociation of enzyme dimer and unfolding of the enzyme at 0.8 mM, but no aggregation at 25°C or 40°C even at high protein concentrations, inactivation kinetics	Oryctolagus cuniculus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	at 5 mM MgCl2	Oryctolagus cuniculus
NaCl	induces folding of the enzyme unfolded by lactic acid, formation of the molten globule conformation with a compact structure	Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + creatine	Oryctolagus cuniculus	-	ADP + phosphocreatine	-	r

Organism

Organism	UniProt	Comment	Textmining
Oryctolagus cuniculus	-	-	-

Renatured (Commentary)

Renatured (Comment)	Organism
enzyme, inactivated due to unfolding after treatment with lactic acid, refolds in presence of NaCl	Oryctolagus cuniculus

Source Tissue

Source Tissue	Comment	Organism	Textmining
skeletal muscle	-	Oryctolagus cuniculus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + creatine	-	Oryctolagus cuniculus	ADP + phosphocreatine	-	r

Subunits

Subunits	Comment	Organism
dimer	-	Oryctolagus cuniculus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	assay at	Oryctolagus cuniculus

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Oryctolagus cuniculus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)