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Literature summary for 2.7.3.2 extracted from

  • Pilla, C.; Cardozo, R.F.; Dornelles, P.K.; Dutra-Filho, C.S.; Wyse, A.T.; Wajner, M.; Wannmacher, C.M.
    Kinetic studies on the inhibition of creatine kinase activity by branched-chain alpha-amino acids in the brain cortex of rats (2003), Int. J. Dev. Neurosci., 21, 145-151.
    View publication on PubMed
Search for more literature for 2.7.3.2

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxymercuribenzoic acid
-
 Rattus norvegicus
L-isoleucine branched chain alpha-amino acids bind at the active site, competitive inhibition mechanism against substrates phosphocreatine and ADP, inhibition kinetics Rattus norvegicus
L-leucine branched chain alpha-amino acids bind at the active site, competitive inhibition mechanism against substrates phosphocreatine and ADP, inhibition kinetics Rattus norvegicus
L-valine branched chain alpha-amino acids bind at the active site, competitive inhibition mechanism against substrates phosphocreatine and ADP, inhibition kinetics Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Rattus norvegicus
0.3
-
 ADP pH 7.5, 37°C Rattus norvegicus
0.8
-
 phosphocreatine pH 7.5, 37°C Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + creatine Rattus norvegicus key enzyme of energy homeostasis ADP + phosphocreatine
-
 r
additional information Rattus norvegicus enzyme inhibition, e.g. by branched chain alpha-amino acids, might contribute to the brain damage maple syrup urine disease MSUD ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 21-day-old Wistar rats
-

Source Tissue

Source Tissue Comment Organism Textmining
brain cortex
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + creatine
-
 Rattus norvegicus ADP + phosphocreatine
-
 r
ATP + creatine key enzyme of energy homeostasis Rattus norvegicus ADP + phosphocreatine
-
 r
additional information enzyme inhibition, e.g. by branched chain alpha-amino acids, might contribute to the brain damage maple syrup urine disease MSUD Rattus norvegicus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ADP
-
 Rattus norvegicus
ATP
-
 Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
10
-
 L-isoleucine versus phosphocreatine, pH 7.5, 37°C Rattus norvegicus
14
-
 L-valine versus phosphocreatine, pH 7.5, 37°C Rattus norvegicus
16
-
 L-leucine versus phosphocreatine, pH 7.5, 37°C Rattus norvegicus
21
-
 L-isoleucine versus ADP, pH 7.5, 37°C Rattus norvegicus
22
-
 L-leucine versus ADP, pH 7.5, 37°C Rattus norvegicus
23
-
 L-valine versus ADP, pH 7.5, 37°C Rattus norvegicus