Literature summary for 2.7.3.2 extracted from

Awama, A.M.; Mazon, H.; Vial, C.; Marcillat, O.
Despite its high similarity with monomeric arginine kinase, muscle creatine kinase is only enzymatically active as a dimer (2007), Arch. Biochem. Biophys., 458, 158-166.

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Protein Variants

Protein Variants	Comment	Organism
W210Y	mutation in the interface of enzyme dimer, dissociates more readily than wild-type to monomer. Dissociation equilibrium constant is 9.7 nM compared to 0.017 nM for wild-type	Oryctolagus cuniculus

General Stability

General Stability	Organism
when incubated with 0.8 M guanidine hydrochloride, MM-CK accumulates as a monomeric molten globule which totally lost its enzymatic activity	Oryctolagus cuniculus

Inhibitors

Inhibitors	Comment	Organism	Structure
guanidine hydrochloride	in the absence of added guanidine hydrochloride, MM-CK activity slightly decreases with NaCl concentration up to 4 M, but a dramatic decline is observed above that value, with full inactivation at 4.5 M. When guanidine is added, curves with similar shapes are obtained but NaCl concentrations needed to inactivate the enzyme are shifted towards lower values	Oryctolagus cuniculus
Guanidinium chloride	inhibitory, in presence of NaCl, increased inhibitory activity. Inactivation by NaCl is due to dissociation of dimeric creatine kinase into its constitutive subunits, and upon monomerization, the protein becomes more susceptible to guanidinium denaturing effect	Oryctolagus cuniculus
NaCl	enzyme activity slightly decreases with NaCl concentration up to 4 M, and a dramatic decline is observed above that value, with full inactivation at 4.5 M. In presence of guanidinium chloride, inactivation occurs much earlier. Inactivation by NaCl is due to dissociation of dimeric creatine kinase into its constitutive subunits, and upon monomerization, the protein becomes more susceptible to guanidinium denaturing effect; in the absence of added guanidine hydrochloride, MM-CK activity slightly decreases with NaCl concentration up to 4 M, but a dramatic decline is observed above that value, with full inactivation at 4.5 M. When guanidine is added, curves with similar shapes are obtained but NaCl concentrations needed to inactivate the enzyme are shifted towards lower values	Oryctolagus cuniculus

Organism

Organism	UniProt	Comment	Textmining
Oryctolagus cuniculus	P00563	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
muscle	-	Oryctolagus cuniculus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + creatine	-	Oryctolagus cuniculus	ADP + phosphocreatine	-	?

Subunits

Subunits	Comment	Organism
dimer	muscle creatine kinase is only enzymatically active as a dimer	Oryctolagus cuniculus

Synonyms

Synonyms	Comment	Organism
MM-CK	-	Oryctolagus cuniculus

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Release 2023.1 (January 2023)