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Literature summary for 2.7.3.2 extracted from

  • Feng, S.; Zhao, T.J.; Zhou, H.M.; Yan, Y.B.
    Effects of the single point genetic mutation D54G on muscle creatine kinase activity, structure and stability (2007), Int. J. Biochem. Cell Biol., 39, 392-401.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D54G mutation identified in acute myocardial infarction patient, mutant shows substantially decreased activity, substrate binding affinity and stability Homo sapiens
D54G the mutant enzyme has substantially decreased activity, substrate binding affinity and stability. Spectroscopic experiments indicate that the mutation impairs the structure of creatine kinase, which results in a partially unfolded state with more hydrophobic exposure and exposed Trp residues. The inability to fold to the functional compact state makes the mutant be prone to aggregate upon microenvironmental stresses, and might gradually decrease the creatine kinase level of the acute myocardial infarction patient Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.46
-
ATP wild-type enzyme Homo sapiens
0.46
-
ATP wild-type Homo sapiens
2.2
-
ATP mutant D54G Homo sapiens
2.2
-
ATP mutant enzyme D54G Homo sapiens
9.6
-
Creatine wild-type enzyme Homo sapiens
9.6
-
Creatine wild-type Homo sapiens
34
-
Creatine mutant D54G Homo sapiens
34
-
Creatine mutant enzyme D54G Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
acute myocardial infarction patient
-

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
52
-
mutant D54G Homo sapiens
52
-
mutant enzyme D54G Homo sapiens
245.5
-
wild-type enzyme Homo sapiens
246
-
wild-type Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + creatine
-
Homo sapiens ADP + phosphocreatine
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
36
-
mutant D54G, melting temperature Homo sapiens
37
-
midpoint temperature of thermal inactivation of mutant enzyme D54G is 36.5°C Homo sapiens
45
-
10 min, mutant enzyme D54G is completely inactivated Homo sapiens
48
-
wild-type, stable up to 48°C Homo sapiens
48
-
10 min, the activity of wild-type enzyme has no significant changes after heat treatment at temperatures below 48°C Homo sapiens
52
-
wild-type, melting temperature Homo sapiens
53
-
midpoint temperature of thermal inactivation of wild-type enzyme is 52.5°C Homo sapiens
58
-
wild-type, complete loss of activity Homo sapiens
58
-
10 min, complete loss of activity above Homo sapiens