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Literature summary for 2.7.3.3 extracted from

  • Pereira, C.A.; Alonso, G.D.; Paveto, M.C.; Iribarren, A.; Cabanas, M.L.; Torres, H.N.; Flawia, M.M.
    Trypanosoma cruzi arginine kinase characterization and cloning. A novel energetic pathway in protozoan parasites (2000), J. Biol. Chem., 275, 1495-1501.
    View publication on PubMed
Search for more literature for 2.7.3.3

Application

Application Comment Organism
medicine arginine kinase is a possible chemotherapy target for Chagas‘ disease Trypanosoma cruzi

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Trypanosoma cruzi

Inhibitors

Inhibitors Comment Organism Structure
agmatine 5fold higher concentration than L-Arg, 20% inhibition Trypanosoma cruzi
canavanine 5fold higher concentration than L-Arg, 50% inhibition Trypanosoma cruzi
Ethylguanidine 5fold higher concentration than L-Arg, 22% inhibition Trypanosoma cruzi
His 5fold higher concentration than L-Arg, 50% inhibition Trypanosoma cruzi
L-Asp 5fold higher concentration than L-Arg, 25% inhibition Trypanosoma cruzi
L-canavanine
-
 Trypanosoma cruzi
L-Glu 5fold higher concentration than L-Arg, 31% inhibition Trypanosoma cruzi
L-homoarginine 5fold higher concentration than L-Arg, 33% inhibition Trypanosoma cruzi
L-Lys 5fold higher concentration than L-Arg, 25% inhibition Trypanosoma cruzi
L-nitroarginine 5fold higher concentration than L-Arg, 28% inhibition Trypanosoma cruzi
N-methyl-L-Arg 5fold higher concentration than L-Arg, 28% inhibition Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 ATP 30°C, pH 7.3 Trypanosoma cruzi
0.3
-
 L-Arg 30°C, pH 7.3 Trypanosoma cruzi

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ a divalent cation such as Mg2+, Mn2+ or Ca2+ is required Trypanosoma cruzi
Mg2+ a divalent cation such as Mg2+, Mn2+ or Ca2+ is required Trypanosoma cruzi
Mn2+ a divalent cation such as Mg2+, Mn2+ or Ca2+ is required Trypanosoma cruzi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40201
-
 x * 40201, calculation from nucleotide sequence Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi O96507
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Arg
-
 Trypanosoma cruzi ADP + Nomega-phospho-L-Arg
-
 ?

Subunits

Subunits Comment Organism
? x * 40201, calculation from nucleotide sequence Trypanosoma cruzi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
 Trypanosoma cruzi

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6
-
 L-canavanine pH 7.3, 30°C Trypanosoma cruzi
7
-
 L-homoarginine pH 7.3, 30°C Trypanosoma cruzi

pI Value

Organism Comment pI Value Maximum pI Value
Trypanosoma cruzi theoretical value calculated from amino acid sequence
-
 7