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Literature summary for 2.7.3.3 extracted from

  • Wu, Q.Y.; Li, F.; Wang, X.Y.; Xu, K.L.
    Evidence that the amino acid residue Ile121 is involved in arginine kinase activity and structural stability (2012), Int. J. Biol. Macromol., 51, 369-377.
    View publication on PubMed
Search for more literature for 2.7.3.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Codon Plus cells Locusta migratoria manilensis

Protein Variants

Protein Variants Comment Organism
I121D the mutation leads to pronounced loss of activity and structural stability. The mutation also leads to serious aggregation during heat-and guanidine hydrochloride-induced denaturation and refolding from the guanidine hydrochloride-denatured state Locusta migratoria manilensis
I121G the mutation leads to pronounced loss of activity and structural stability. The mutation also leads to serious aggregation during heat-and guanidine hydrochloride-induced denaturation and refolding from the guanidine hydrochloride-denatured state Locusta migratoria manilensis
I121K the mutation leads to pronounced loss of activity and structural stability. The mutation also leads to serious aggregation during heat-and guanidine hydrochloride-induced denaturation and refolding from the guanidine hydrochloride-denatured state Locusta migratoria manilensis
I121L the almost has no effect on AK activity and structural stability Locusta migratoria manilensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.94
-
 L-Arg native wild type enzyme, in 100 mM Tris, pH 8.0, at 30°C Locusta migratoria manilensis
0.951
-
 L-Arg recombinant wild type enzyme, in 100 mM Tris, pH 8.0, at 30°C Locusta migratoria manilensis
0.98
-
 L-Arg mutant enzyme I121L, in 100 mM Tris, pH 8.0, at 30°C Locusta migratoria manilensis
1.27
-
 ATP recombinant wild type enzyme, in 100 mM Tris, pH 8.0, at 30°C Locusta migratoria manilensis
1.29
-
 ATP native wild type enzyme, in 100 mM Tris, pH 8.0, at 30°C Locusta migratoria manilensis
1.36
-
 ATP mutant enzyme I121L, in 100 mM Tris, pH 8.0, at 30°C Locusta migratoria manilensis
1.74
-
 L-Arg mutant enzyme I121G, in 100 mM Tris, pH 8.0, at 30°C Locusta migratoria manilensis
2.38
-
 ATP mutant enzyme I121G, in 100 mM Tris, pH 8.0, at 30°C Locusta migratoria manilensis
2.5 3 L-Arg mutant enzyme I121K, in 100 mM Tris, pH 8.0, at 30°C Locusta migratoria manilensis
3.04
-
 L-Arg mutant enzyme I121D, in 100 mM Tris, pH 8.0, at 30°C Locusta migratoria manilensis
3.56
-
 ATP mutant enzyme I121K, in 100 mM Tris, pH 8.0, at 30°C Locusta migratoria manilensis
4.06
-
 ATP mutant enzyme I121D, in 100 mM Tris, pH 8.0, at 30°C Locusta migratoria manilensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 x * 40000, calculated from amino acid sequence Locusta migratoria manilensis

Organism

Organism UniProt Comment Textmining
Locusta migratoria manilensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Locusta migratoria manilensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Arg
-
 Locusta migratoria manilensis ADP + Nomega-phospho-L-Arg
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, calculated from amino acid sequence Locusta migratoria manilensis

Synonyms

Synonyms Comment Organism
ATP: L-arginine phosphotransferase
-
 Locusta migratoria manilensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45 65 the wild type enzyme can retain its activity well at temperatures lower than 48°C, and then its activity shows a steep decrease from 45 to 60°C and completely loses its activity at temperatures above 65°C. The melting temperature is at about 56°C Locusta migratoria manilensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
60.36
-
 L-Arg mutant enzyme I121D, in 100 mM Tris, pH 8.0, at 30°C Locusta migratoria manilensis
70.93
-
 L-Arg mutant enzyme I121K, in 100 mM Tris, pH 8.0, at 30°C Locusta migratoria manilensis
109.8
-
 L-Arg mutant enzyme I121G, in 100 mM Tris, pH 8.0, at 30°C Locusta migratoria manilensis
151.2
-
 L-Arg mutant enzyme I121L, in 100 mM Tris, pH 8.0, at 30°C Locusta migratoria manilensis
159.4
-
 L-Arg recombinant wild type enzyme, in 100 mM Tris, pH 8.0, at 30°C Locusta migratoria manilensis
163
-
 L-Arg native wild type enzyme, in 100 mM Tris, pH 8.0, at 30°C Locusta migratoria manilensis