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Literature summary for 2.7.3.5 extracted from
- The purification and properties of adenosine triphosphate-lombricine phosphotransferase (1964), Biochem. J., 90, 170-176.
General Stability
| General Stability | Organism |
|---|---|
| freeze-drying of preparations in non-volatile buffers causes almost 40% loss of activity. Freezing and thawing at pH 8.6 in N-ethylmorpholine-hydrochloric acid buffer produces complete inactivation | Megascolides cameroni |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NEM | 0.3 mM, complete inhibition | Megascolides cameroni |  |
| o-Iodosobenzoate | 0.3 mM, complete inhibition | Megascolides cameroni | |
| p-hydroxymercuribenzoate | 0.0001 mM, complete inhibition | Megascolides cameroni | |
| Phenyl iodoacetate | 1 mM, complete inhibition | Megascolides cameroni | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.4 | - |
phospholombricine | pH 8.6, 30°C | Megascolides cameroni | |
| 9.1 | - |
D-lombricine | pH 8.6, 30°C | Megascolides cameroni | |
| 11.7 | - |
L-lombricine | pH 8.6, 30°C | Megascolides cameroni | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 5 mM, slight activation in both directions | Megascolides cameroni | |
| Co2+ | 5 mM, slight activation in both directions | Megascolides cameroni | |
| Mg2+ | 5 mM, activation in both directions | Megascolides cameroni | |
| Mn2+ | 5 mM, activation in both directions | Megascolides cameroni | |
| additional information | not activated by Ni2+, Cu2+, Fe2+, Fe3+, Al3+, Sn2+, Ba2+, Cd2+, Zn2+, Be2+ | Megascolides cameroni |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Megascolides cameroni | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Megascolides cameroni |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| muscle | - |
Megascolides cameroni | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 31 | - |
D-lombricine | Megascolides cameroni |
| 49 | - |
L-lombricine | Megascolides cameroni |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 2°C, pH 7.5, 50% v/v glycerol, several months stable | Megascolides cameroni |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-lombricine | - |
Megascolides cameroni | ADP + phospholombricine | - |
r | |
| ATP + L-lombricine | - |
Megascolides cameroni | ADP + phospholombricine | - |
r | |
| ATP + lombricine | i.e. guanidinoethylphospho-O-serine | Megascolides cameroni | ADP + phospholombricine | - |
r | |
| dADP + phospholombricine | at 16% of the activity with ADP | Megascolides cameroni | dATP + lombricine | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
synthesis of ATP | Megascolides cameroni |
| 8.6 | - |
phosphorylation of lombricine | Megascolides cameroni |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8 | pH 6.5: about 85% of maximal activity, pH 8.0: about 50% of maximal activity, synthesis of ATP | Megascolides cameroni |
| 7.5 | 9.5 | pH 7.5: about 65% of maximal activity, pH 9.5: about 45% of maximal activity, phosphorylation of lombricine | Megascolides cameroni |
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