KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | 0.031 | histidine-containing protein | 37°C, pH 7.0 | Streptococcus salivarius | |
0.47 | 0.48 | phosphoenolpyruvate | 37°C, pH 7.0 | Streptococcus salivarius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
135000 | - |
size exclusion chromatography | Streptococcus salivarius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus salivarius | - |
immunologically distinct from Escherichia coli or other Streptococci | - |
Purification (Comment) | Organism |
---|---|
- |
Streptococcus salivarius |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
phosphoenolpyruvate + protein histidine = pyruvate + protein Npi-phospho-L-histidine | ping-pong mechanism | Streptococcus salivarius |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
530 | - |
37°C, pH 7.0 | Streptococcus salivarius |
Storage Stability | Organism |
---|---|
-20°C, 10 mM potassium phosphate buffer, pH 7.5, 0.1 M KCl, 20% glycerol, no significant loss of activity in several months | Streptococcus salivarius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + histidine-containing protein | - |
Streptococcus salivarius | pyruvate + phosphorylated histidine-containing protein | - |
? |