Literature summary for 2.7.3.9 extracted from

Weigel, N.; Waygood, E.B.; Kukuruzinska, M.A.; Nakazawa, A.; Roseman, S.
Sugar transport by the bacterial phosphotransferase system. Isolation and characterization of enzyme I from Salmonella typhimurium (1982), J. Biol. Chem., 257, 14461-14469.

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0054	-	histidine-containing protein	-	Salmonella enterica subsp. enterica serovar Typhimurium
0.195	0.2	phosphoenolpyruvate	37°C, pH 7.2	Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
130000	150000	gel filtration at room temperature	Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism	UniProt	Comment	Textmining
Salmonella enterica subsp. enterica serovar Typhimurium	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
no glycoprotein	-	Salmonella enterica subsp. enterica serovar Typhimurium

Purification (Commentary)

Purification (Comment)	Organism
-	Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
8929	-	-	Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

Storage Stability	Organism
-18°C, 0.1 M potassium phosphate buffer, or 0.01 M Tris buffer, pH 6.5, 1 mM EDTA, 0.5 mM dithioerythritol, 5 mM MgCl2, 2-3 months stable	Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphoenolpyruvate + histidine-containing protein	-	Salmonella enterica subsp. enterica serovar Typhimurium	pyruvate + phosphorylated histidine-containing protein	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 57000-70000, gel filtration at 4-6°C	Salmonella enterica subsp. enterica serovar Typhimurium
dimer	2 * 57000-70000, SDS-PAGE	Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	7.5	-	Salmonella enterica subsp. enterica serovar Typhimurium
6.7	-	-	Salmonella enterica subsp. enterica serovar Typhimurium

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Release 2023.1 (January 2023)