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Literature summary for 2.7.3.9 extracted from
- Enzyme I from Salmonella typhimurium (1982), Methods Enzymol., 90, 431-436.
No PubMed abstract available
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
General Stability
| General Stability | Organism |
|---|---|
| labile in purified state, sensitive to sulfhydryl reagents | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium |  |
| sulfhydryl reagents | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| Zn2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0054 | - |
histidine-containing protein | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.195 | 0.2 | phosphoenolpyruvate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | Km: 0.05 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
| Mg2+ | Km: 0.53 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
| Mn2+ | Km: 0.05 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
monomer, gel filtration at 4-6°C | Salmonella enterica subsp. enterica serovar Typhimurium |
| 130000 | 150000 | gel filtration at room temperature | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| phosphoenolpyruvate + protein histidine = pyruvate + protein Npi-phospho-L-histidine | bi-bi ping-pong mechanism | Salmonella enterica subsp. enterica serovar Typhimurium | |
| phosphoenolpyruvate + protein histidine = pyruvate + protein Npi-phospho-L-histidine | in a first reaction step phosphate is transferred to the N-3 position of the imidazole ring of histidine | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8929 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -18°C, 0.05 M potassium phosphate buffer, pH 6.5, 0.2 mM dithioerythritol, 1 mM EDTA, several months | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + histidine-containing protein | in a first reaction step phosphate is transferred to the N-3 position of the imidazole ring of histidine | Salmonella enterica subsp. enterica serovar Typhimurium | pyruvate + phosphorylated histidine-containing protein | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 57000-70000, gel filtration under denaturing conditions | Salmonella enterica subsp. enterica serovar Typhimurium |
| dimer | 2 * 57000-70000, sedimentation equilibrium centrifugation | Salmonella enterica subsp. enterica serovar Typhimurium |
| dimer | 2 * 57000-70000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.7 | - |
Co2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
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