Literature summary for 2.7.3.9 extracted from

Navdaeva, V.; Zurbriggen, A.; Waltersperger, S.; Schneider, P.; Oberholzer, A.; Baehler, P.; Baechler, C.; Grieder, A.; Baumann, U.; Erni, B.
Phosphoenolpyruvate: sugar phosphotransferase system from the hyperthermophilic Thermoanaerobacter tengcongensis (2011), Biochemistry, 50, 1184-1193.

Search for more literature for 2.7.3.9

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli K-12 and Rosetta (DE3) cells	Caldanaerobacter subterraneus subsp. tengcongensis

Crystallization (Commentary)

Crystallization (Comment)	Organism
C-terminal domain of enzyme I in complex with phosphoenolpyruvate, sitting drop vapor diffusion method, using 0.1 M succinic acid (pH 7.0), and 15% PEG3350 at 20°C	Caldanaerobacter subterraneus subsp. tengcongensis

Protein Variants

Protein Variants	Comment	Organism
C502A	active site mutant	Caldanaerobacter subterraneus subsp. tengcongensis
H189A	active site mutant	Caldanaerobacter subterraneus subsp. tengcongensis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38165	-	x * 38165, electrospray ionization mass spectrometry	Caldanaerobacter subterraneus subsp. tengcongensis

Organism

Organism	UniProt	Comment	Textmining
Caldanaerobacter subterraneus subsp. tengcongensis	Q8R7R4	-	-
Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 / JCM 11007 / NBRC 100824 / MB4	Q8R7R4	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	phosphorylation of the N-terminal domain of enzyme I at His189 is catalyzed by the C-terminal domain of enzyme I	Caldanaerobacter subterraneus subsp. tengcongensis

Purification (Commentary)

Purification (Comment)	Organism
Ni2+-NTA column chromatography and gel filtration	Caldanaerobacter subterraneus subsp. tengcongensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphoenolpyruvate + protein histidine	-	Caldanaerobacter subterraneus subsp. tengcongensis	pyruvate + protein Npi-phospho-L-histidine	-	?
phosphoenolpyruvate + protein histidine	-	Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 / JCM 11007 / NBRC 100824 / MB4	pyruvate + protein Npi-phospho-L-histidine	-	?

Subunits

Subunits	Comment	Organism
?	x * 38165, electrospray ionization mass spectrometry	Caldanaerobacter subterraneus subsp. tengcongensis

Synonyms

Synonyms	Comment	Organism
EIC	C-terminal domain of enzyme I	Caldanaerobacter subterraneus subsp. tengcongensis
More	enzyme I is part of the phosphoenolpyruvate: sugar phosphotransferase system	Caldanaerobacter subterraneus subsp. tengcongensis
ptsA	-	Caldanaerobacter subterraneus subsp. tengcongensis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)