Activating Compound | Comment | Organism | Structure |
---|---|---|---|
basic protein | activation | Sinomonas atrocyanea | |
basic protein | can substitute for histone | Sinomonas atrocyanea | |
bovine serum albumin | activation, can substitute for histone only in the absence of phosphate | Sinomonas atrocyanea | |
casein | activation, can substitute for histone only in the absence of phosphate | Sinomonas atrocyanea | |
histone | required for activity | Sinomonas atrocyanea | |
phosphate | - |
Sinomonas atrocyanea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | 4 mM, product inhibition | Sinomonas atrocyanea | |
ATP | free form, above 0.008 mM | Sinomonas atrocyanea | |
diphosphate | 1 mM, complete inhibition | Sinomonas atrocyanea | |
F- | 20 mM, complete inhibition | Sinomonas atrocyanea | |
Mg2+ | free form, above 6 mM, activating below | Sinomonas atrocyanea | |
Mn2+ | free form, above 2 mM, activating below | Sinomonas atrocyanea | |
MnATP2- | at high concentrations, above 0.6 mM, activating below | Sinomonas atrocyanea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.53 | - |
ATP | pH 7.0, 30°C | Sinomonas atrocyanea | |
0.53 | - |
ATP | ATP in form of MnATP2- | Sinomonas atrocyanea | |
1.67 | - |
phosphate | pH 7.0, 30°C | Sinomonas atrocyanea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Sinomonas atrocyanea | |
Mg2+ | inhibitory above 6 mM | Sinomonas atrocyanea | |
Mg2+ | active substrate: MgATP | Sinomonas atrocyanea | |
Mn2+ | activation, can replace Mg2+ | Sinomonas atrocyanea | |
Mn2+ | maximal activity at 1.0-2.0 mM, inhibition above 2 mM | Sinomonas atrocyanea | |
Mn2+ | active substrate: MnATP | Sinomonas atrocyanea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sinomonas atrocyanea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DEAE-cellulose, Sephadex G-200 | Sinomonas atrocyanea |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.82 | - |
- |
Sinomonas atrocyanea |
Storage Stability | Organism |
---|---|
5°C, at least 6 months | Sinomonas atrocyanea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (phosphate)n | incorporates gamma-phosphate of ATP into long-chain polyphosphate molecules | Sinomonas atrocyanea | ADP + (phosphate)n+1 | - |
? | |
ATP + phosphate | - |
Sinomonas atrocyanea | ADP + diphosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Sinomonas atrocyanea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | - |
Sinomonas atrocyanea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | approx. half-maximal activity at pH 5.0 and pH 8.0 | Sinomonas atrocyanea |