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Literature summary for 2.7.4.1 extracted from
- Orthophosphate and histone dependent polyphosphate kinase from E. coli (1973), Biochem. Biophys. Res. Commun., 53, 875-881.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| basic protein | activation | Escherichia coli | |
| bovine serum albumin | activation, can substitute for histone only in the absence of phosphate | Escherichia coli | |
| casein | activation, can substitute for histone only in the absence of phosphate | Escherichia coli |  |
| histone | strong inhibition of reverse reaction | Escherichia coli | |
| histone | maximum activation in the presence of 20 mM phosphate | Escherichia coli | |
| phosphate | inhibiton above 20 mM | Escherichia coli | |
| phosphate | required for activity, only in the presence of histone | Escherichia coli | |
| protamine | required for maximal activity | Escherichia coli | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | 0.2 mM, complete inhibition | Escherichia coli | |
| diphosphate | 1 mM, complete inhibition | Escherichia coli | |
| F- | 20 mM, complete inhibition | Escherichia coli | |
| histone | reverse reaction, strong, activates forward reaction in the presence of phosphate | Escherichia coli | |
| KCl | - |
Escherichia coli | |
| additional information | not inhibited by 3',5'-adenosine monophosphate | Escherichia coli | |
| NaCl | - |
Escherichia coli | |
| phosphate | above 20 mM; activates in the presence of histone | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.4 | - |
ATP | pH 7.0, 30°C | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activation | Escherichia coli | |
| Mn2+ | activation, can replace Mg2+ | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (phosphate)n | Escherichia coli | involved in phosphate metabolism of bacteria | ADP + (phosphate)n+1 | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
strain K-12, Crooke's strain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, DEAE-cellulose, Sephadex G-200 | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.15 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (phosphate)n | - |
Escherichia coli | ADP + (phosphate)n+1 | - |
? | |
| ATP + (phosphate)n | involved in phosphate metabolism of bacteria | Escherichia coli | ADP + (phosphate)n+1 | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Escherichia coli |
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Release 2023.1 (January 2023)
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