Activating Compound | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | activation, up to 100 mM, inhibition at higher concentrations | Escherichia coli | |
Tetrapolyphosphate | activation, removes lag-phase in synthesis at low ATP-levels, not phosphate, diphosphate or tripolyphosphate | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | activation up to 100 mM; inhibition at higher concentrations; strong inhibition | Escherichia coli | |
ADP | 50%, 70% and 93% inhibition at 0.08 mM, 0.15 mM and 0.2 mM ADP, respectively | Escherichia coli | |
KCl | 50 mM, 50% inhibition | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
ATP | pH 7.2, 37°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activation | Escherichia coli | |
Mg2+ | maximal activity at 5-10 mM at 1 mM ATP | Escherichia coli | |
Mn2+ | activation, can replace Mg2+ | Escherichia coli | |
Mn2+ | 2 mM, 22% of activation with Mg2+ | Escherichia coli | |
additional information | not activated by Ca2+ | Escherichia coli | |
Zn2+ | activation, 0.4 mM, 10% as effective as Mg2+ | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69000 | - |
4 * 69000, SDS-PAGE | Escherichia coli |
266000 | - |
gel filtration | Escherichia coli |
270000 | - |
glycerol density gradient centrifugation | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, phosphocellulose, DEAE-cellulose, Mono S | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.003 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (phosphate)n | autophosphorylation, i.e. the gamma-phosphate of ATP becomes covalently attached to the enzyme under condition of polyphosphate synthesis, 0.52-0.92 mol phosphate per mol enzyme | Escherichia coli | ADP + (phosphate)n+1 | via phosphorylated enzyme intermediate | ? | |
ATP + (phosphate)n | no primer substrate required | Escherichia coli | ADP + (phosphate)n+1 | via phosphorylated enzyme intermediate | ? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 69000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
23.6 | - |
ATP | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
ADP | pH 7.2, 37°C | Escherichia coli |