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Literature summary for 2.7.4.1 extracted from

  • Ahn, K.; Kornberg, A.
    Polyphosphate kinase from Escherichia coli. Purification and demonstration of a phosphoenzyme intermediate (1990), J. Biol. Chem., 265, 11734-11739.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
(NH4)2SO4 activation, up to 100 mM, inhibition at higher concentrations Escherichia coli
Tetrapolyphosphate activation, removes lag-phase in synthesis at low ATP-levels, not phosphate, diphosphate or tripolyphosphate Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4 activation up to 100 mM; inhibition at higher concentrations; strong inhibition Escherichia coli
ADP 50%, 70% and 93% inhibition at 0.08 mM, 0.15 mM and 0.2 mM ADP, respectively Escherichia coli
KCl 50 mM, 50% inhibition Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
ATP pH 7.2, 37°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activation Escherichia coli
Mg2+ maximal activity at 5-10 mM at 1 mM ATP Escherichia coli
Mn2+ activation, can replace Mg2+ Escherichia coli
Mn2+ 2 mM, 22% of activation with Mg2+ Escherichia coli
additional information not activated by Ca2+ Escherichia coli
Zn2+ activation, 0.4 mM, 10% as effective as Mg2+ Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69000
-
4 * 69000, SDS-PAGE Escherichia coli
266000
-
gel filtration Escherichia coli
270000
-
glycerol density gradient centrifugation Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, phosphocellulose, DEAE-cellulose, Mono S Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.003
-
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (phosphate)n autophosphorylation, i.e. the gamma-phosphate of ATP becomes covalently attached to the enzyme under condition of polyphosphate synthesis, 0.52-0.92 mol phosphate per mol enzyme Escherichia coli ADP + (phosphate)n+1 via phosphorylated enzyme intermediate ?
ATP + (phosphate)n no primer substrate required Escherichia coli ADP + (phosphate)n+1 via phosphorylated enzyme intermediate ?

Subunits

Subunits Comment Organism
tetramer 4 * 69000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
23.6
-
ATP
-
Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.09
-
ADP pH 7.2, 37°C Escherichia coli