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Literature summary for 2.7.4.1 extracted from
- Use of an Escherichia coli recombinant producing thermostable polyphosphate kinase as an ATP regenerator to produce fructose 1,6-diphosphate (2007), Appl. Environ. Microbiol., 73, 5676-5678.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the enzyme is useful for ATP production from polyphosphate | Thermus thermophilus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Thermus thermophilus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Thermus thermophilus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (phosphate)n | Thermus thermophilus | - |
ADP + (phosphate)n+1 | - |
r | |
| ATP + (phosphate)n | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | - |
ADP + (phosphate)n+1 | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus | Q72JY1 | - |
- |
| Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | Q72JY1 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (phosphate)n | - |
Thermus thermophilus | ADP + (phosphate)n+1 | - |
r | |
| ATP + (phosphate)n | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | ADP + (phosphate)n+1 | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| polyP kinase | - |
Thermus thermophilus |
| PPK | - |
Thermus thermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
assay at | Thermus thermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
recombinant enzyme in Escherichia coli cells, 10 min, then mixed with 50 mM MOPS, pH 6.0, containing 0.08 mM ADP, 40 mM (NH4)2SO4, 4 mM MgCl2, and 5 mM polyP65, the activity is detected in the precipitate. After one week, 60% of the activity is still remaining | Thermus thermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Thermus thermophilus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Thermus thermophilus | |
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Release 2023.1 (January 2023)
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