Activating Compound | Comment | Organism | Structure |
---|---|---|---|
actin | Dictyostelium discoideum also has an actin-related poly P synthesizing complex, DdPPK2, that is KCl dependent | Dictyostelium discoideum | |
additional information | polyphosphate or ATP promote oligomerization of the enzyme in vitro, polyphosphate activates about 10fold | Dictyostelium discoideum |
Cloned (Comment) | Organism |
---|---|
gene Ddppk1, overexpression under control of the strong promoter Pact15, functional expression of GFP-DdPPK1 fusion protein | Dictyostelium discoideum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | DdPPK1 mutants show reduced polyphosphate levels and are defective in fruiting body development, sporulation, and predation, and in late stages of cytokinesis and cell division, overview. The enzyme-defective mutants do not produce polyphosphate exopolymer | Dictyostelium discoideum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | inhibits PPK1 80% at 500 mM, but not PPK2 | Dictyostelium discoideum | |
phalloidin | a heptapeptide toxin from the mushroom Amanita phalloides, which binds tightly and specifically to polymerized actin, inhibits DdPPK2 and DdPPK1, the latter by 80% at 10 nM, the molar ratio of phalloidin to DdPPK1 of 10:15 results in complete inhibition of DdPPK1 activity | Dictyostelium discoideum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | vesicles | Dictyostelium discoideum | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | Dictyostelium discoideum also has an actin-related poly P synthesizing complex, DdPPK2, that is KCl dependent | Dictyostelium discoideum | |
Mg2+ | - |
Dictyostelium discoideum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
118000 | - |
6 * 118000, SDS-PAGE, DdPPK1 forms higher oligomeric structures in the presence of either poly phosphate or ATP | Dictyostelium discoideum |
720000 | - |
gel filtration | Dictyostelium discoideum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (phosphate)n | Dictyostelium discoideum | PPK1 is the principal enzyme responsible for reversible synthesis of polyphosphate from the terminal phosphate of ATP | ADP + (phosphate)n+1 | - |
r | |
additional information | Dictyostelium discoideum | the organism also has an actin-related poly P synthesizing complex, DdPPK2, that is KCl dependent | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyostelium discoideum | Q54BM7 | gene Ddppk1 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | DdPPK1 contains the conserved residues for autophosphorylation | Dictyostelium discoideum |
Purification (Comment) | Organism |
---|---|
native enzyme 480fold by heparin affinity chromatography and two different steps of anion exchange chromatography | Dictyostelium discoideum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.048 | - |
purified enzyme | Dictyostelium discoideum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (phosphate)n | - |
Dictyostelium discoideum | ADP + (phosphate)n+1 | DdPPK1 products are heterogeneous in chain length, with broad-range chain lengths of 50-300 Pi residues, and shorter compared to those of Escherichia coli | r | |
ATP + (phosphate)n | PPK1 is the principal enzyme responsible for reversible synthesis of polyphosphate from the terminal phosphate of ATP | Dictyostelium discoideum | ADP + (phosphate)n+1 | - |
r | |
additional information | the organism also has an actin-related poly P synthesizing complex, DdPPK2, that is KCl dependent | Dictyostelium discoideum | ? | - |
? | |
additional information | the enzyme from Dictyostelium discoideum performs autophosphorylation and has an unique N-terminal extension of 370 amino acids, lacking homology with any known protein, that is necessary for its enzymatic activity, cellular localization, and physiological functions, overview | Dictyostelium discoideum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 118000, SDS-PAGE, DdPPK1 forms higher oligomeric structures in the presence of either poly phosphate or ATP | Dictyostelium discoideum |
More | the enzyme from Dictyostelium discoideum has an unique N-terminal extension of 370 amino acids, lacking homology with any known protein, that is necessary for its enzymatic activity, cellular localization, and physiological functions, overview | Dictyostelium discoideum |
Synonyms | Comment | Organism |
---|---|---|
plyphosphate kinase 1 | - |
Dictyostelium discoideum |
PPK1 | - |
Dictyostelium discoideum |
PPK2 | - |
Dictyostelium discoideum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Dictyostelium discoideum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 40 | - |
Dictyostelium discoideum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | DdPPK1 contains the conserved residues for ATP binding | Dictyostelium discoideum |