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Literature summary for 2.7.4.1 extracted from

  • Shum, K.T.; Lui, E.L.; Wong, S.C.; Yeung, P.; Sam, L.; Wang, Y.; Watt, R.M.; Tanner, J.A.
    Aptamer-mediated inhibition of Mycobacterium tuberculosis polyphosphate kinase 2 (2011), Biochemistry, 50, 3261-3271.
    View publication on PubMed
Search for more literature for 2.7.4.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
additional information a G9-quadruplex DNA aptamer inhibits isoform PPK2 with an IC50 of 40 nM and exhibits noncompetitive inhibition kinetics Mycobacterium tuberculosis
additional information a G9-quadruplex DNA aptamer inhibits isoform PPK2 with an IC50 of 105 nM and exhibits noncompetitive inhibition kinetics Vibrio cholerae serotype O1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 (phosphate)45 polyphosphate synthesis reaction, in 50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C Mycobacterium tuberculosis
0.0054
-
 (phosphate)65 polyphosphate synthesis reaction, in 50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C Mycobacterium tuberculosis
0.006
-
 (phosphate)45 polyphosphate utilization reaction, in 50 mM Tris-HCl (pH 8.5), 30 mM MgCl2, at 25°C Mycobacterium tuberculosis
0.0063
-
 (phosphate)65 polyphosphate utilization reaction, in 50 mM Tris-HCl (pH 8.5), 30 mM MgCl2, at 25°C Mycobacterium tuberculosis
0.33
-
 ATP 50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C Mycobacterium tuberculosis
0.38
-
 ADP 50 mM Tris-HCl (pH 8.5), 30 mM MgCl2, at 25°C Mycobacterium tuberculosis
0.66
-
 GTP 50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimum activity at 30 mM Mg2+ Mycobacterium tuberculosis
Mn2+ optimum activity at 1 mM Mn2+ Mycobacterium tuberculosis
additional information Fe2+, Zn2+, Cu2+ cannot be used by the enzyme Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-
Vibrio cholerae serotype O1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-charged His-Trap chelating column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + (phosphate)45
-
 Vibrio cholerae serotype O1 ATP + (phosphate)44
-
 r
ADP + (phosphate)45 similar catalytic activities with either (phosphate)65 or (phosphate)45. Isoform PPK2 catalyzes polyphosphate-dependent phosphorylation of ADP to ATP at a rate 838times higher than the rate of polyphosphate synthesis Mycobacterium tuberculosis ATP + (phosphate)44
-
 r
ADP + (phosphate)45 similar catalytic activities with either (phosphate)65 or (phosphate)45. Isoform PPK2 catalyzes polyphosphate-dependent phosphorylation of ADP to ATP at a rate 838times higher than the rate of polyphosphate synthesis Mycobacterium tuberculosis H37Rv ATP + (phosphate)44
-
 r
ADP + (phosphate)65
-
 Vibrio cholerae serotype O1 ATP + (phosphate)64
-
 r
ADP + (phosphate)65 similar catalytic activities with either (phosphate)65 or (phosphate)45. Isoform PPK2 catalyzes polyphosphate-dependent phosphorylation of ADP to ATP at a rate 838times higher than the rate of polyphosphate synthesis Mycobacterium tuberculosis ATP + (phosphate)64
-
 r
ADP + (phosphate)65 similar catalytic activities with either (phosphate)65 or (phosphate)45. Isoform PPK2 catalyzes polyphosphate-dependent phosphorylation of ADP to ATP at a rate 838times higher than the rate of polyphosphate synthesis Mycobacterium tuberculosis H37Rv ATP + (phosphate)64
-
 r
ATP + (phosphate)45
-
 Mycobacterium tuberculosis ADP + (phosphate)46
-
 r
ATP + (phosphate)45
-
 Mycobacterium tuberculosis H37Rv ADP + (phosphate)46
-
 r
ATP + (phosphate)65
-
 Mycobacterium tuberculosis ADP + (phosphate)66
-
 r
ATP + (phosphate)65
-
 Mycobacterium tuberculosis H37Rv ADP + (phosphate)66
-
 r
GTP + (phosphate)65
-
 Mycobacterium tuberculosis GDP + (phosphate)66
-
 ?
additional information the enzyme is unable to use triphosphate or pentaphosphate as phosphate donors Mycobacterium tuberculosis ?
-
 ?
additional information the enzyme is unable to use triphosphate or pentaphosphate as phosphate donors Mycobacterium tuberculosis H37Rv ?
-
 ?

Subunits

Subunits Comment Organism
octamer gel filtration Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
polyphosphate kinase 2
-
 Mycobacterium tuberculosis
polyphosphate kinase 2
-
 Vibrio cholerae serotype O1
polyphosphate-dependent nucleoside diphosphate kinase
-
 Mycobacterium tuberculosis
polyphosphate-dependent nucleoside diphosphate kinase
-
 Vibrio cholerae serotype O1
PPK2
-
 Mycobacterium tuberculosis
PPK2
-
 Vibrio cholerae serotype O1

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
 (phosphate)65 polyphosphate synthesis reaction, in 50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C Mycobacterium tuberculosis
0.02
-
 (phosphate)45 polyphosphate synthesis reaction, in 50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C Mycobacterium tuberculosis
0.05
-
 ATP 50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C Mycobacterium tuberculosis
0.08
-
 GTP 50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C Mycobacterium tuberculosis
41.9
-
 ADP 50 mM Tris-HCl (pH 8.5), 30 mM MgCl2, at 25°C Mycobacterium tuberculosis
50
-
 (phosphate)45 polyphosphate utilization reaction, in 50 mM Tris-HCl (pH 8.5), 30 mM MgCl2, at 25°C Mycobacterium tuberculosis
53
-
 (phosphate)65 polyphosphate utilization reaction, in 50 mM Tris-HCl (pH 8.5), 30 mM MgCl2, at 25°C Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.121
-
 GTP 50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C Mycobacterium tuberculosis
0.15
-
 ATP 50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C Mycobacterium tuberculosis
3.704
-
 (phosphate)65 polyphosphate synthesis reaction, in 50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C Mycobacterium tuberculosis
4
-
 (phosphate)45 polyphosphate synthesis reaction, in 50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C Mycobacterium tuberculosis
110
-
 ADP 50 mM Tris-HCl (pH 8.5), 30 mM MgCl2, at 25°C Mycobacterium tuberculosis
8300
-
 (phosphate)45 polyphosphate utilization reaction, in 50 mM Tris-HCl (pH 8.5), 30 mM MgCl2, at 25°C Mycobacterium tuberculosis
8400
-
 (phosphate)65 polyphosphate utilization reaction, in 50 mM Tris-HCl (pH 8.5), 30 mM MgCl2, at 25°C Mycobacterium tuberculosis