Cloned (Comment) | Organism |
---|---|
His-tagged enzyme is expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
A615S | the mutant exhibits about wild type activity for ATP synthesis and polyphosphate synthesis | Mycobacterium tuberculosis |
E681A | the mutant exhibits less than 5% activity for ATP synthesis and less than 30% activity for polyphosphate synthesis compared o the wild type enzyme | Mycobacterium tuberculosis |
H150A | the mutation causes PPK1 protein deficient but not defective in autophosphorylation and severely affects ATP or polyphosphate synthesis | Mycobacterium tuberculosis |
H150Q | the mutation causes PPK1 protein deficient but not defective in autophosphorylation and mildly affects ATP or polyphosphate synthesis | Mycobacterium tuberculosis |
H480A | the mutant catalytic efficiency for the polyphosphate synthesis is almost or very close to that of the wild type enzyme | Mycobacterium tuberculosis |
H480Q | the mutant catalytic efficiency for the polyphosphate synthesis is almost or very close to that of the wild type enzyme | Mycobacterium tuberculosis |
H491A | inactive | Mycobacterium tuberculosis |
H491Q | inactive | Mycobacterium tuberculosis |
H510Q | the mutant catalytic efficiency for the polyphosphate synthesis is almost or very close to that of the wild type enzyme | Mycobacterium tuberculosis |
N515A | the mutant exhibits less than 2% activity for ATP synthesis and about 65% activity for polyphosphate synthesis compared to the wild type enzyme | Mycobacterium tuberculosis |
R431A | the mutant exhibits less than 5% activity for ATP synthesis and about 78% activity for polyphosphate synthesis compared to the wild type enzyme | Mycobacterium tuberculosis |
R461A | the mutant exhibits less than 2% activity for ATP synthesis and about 76% activity for polyphosphate synthesis compared to the wild type enzyme | Mycobacterium tuberculosis |
R624A | the mutant exhibits less than 2% activity for ATP synthesis and about 65% activity for polyphosphate synthesis compared to the wild type enzyme | Mycobacterium tuberculosis |
R654A | the mutant exhibits less than 1% activity for ATP synthesis and about 90% activity for polyphosphate synthesis compared to the wild type enzyme | Mycobacterium tuberculosis |
S668A | the mutant exhibits less than 5% activity for ATP synthesis and less than 30% activity for polyphosphate synthesis compared to the wild type enzyme | Mycobacterium tuberculosis |
Y524A | the mutant exhibits less than 10% activity for ATP synthesis and less than 20% activity for polyphosphate synthesis compared to the wild type enzyme | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
ADP | isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
0.176 | - |
ADP | mutant H510Q of isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
0.18 | - |
ADP | mutant H480Q of isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
0.2 | - |
ADP | mutant H480A of isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
1.35 | - |
ATP | isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
1.38 | - |
ATP | mutant H480Q of isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
1.47 | - |
ATP | mutant H480A of isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
1.5 | 2 | ATP | mutant H510Q of isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
2 | - |
ADP | mutant Y524A of isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
5.2 | - |
ATP | mutant H510A of isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
6.8 | - |
ADP | mutant H510A of isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
8 | - |
ADP | isoform PPK2, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
11.3 | - |
GDP | isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
13 | - |
ATP | mutant Y524A of isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | necessary for activity | Mycobacterium tuberculosis | |
Mn2+ | necessary for activity | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
87000 | - |
2 * 87000, isoform PPK1, SDS-PAGE | Mycobacterium tuberculosis |
197000 | - |
isoform PPK1, gel filtration | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the protein is capable of autophosphorylation. Only His-491 is autophosphorylated and crucial for any enzymatic activity | Mycobacterium tuberculosis |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + (phosphate)20 | - |
Mycobacterium tuberculosis | ATP + (phosphate)19 | - |
r | |
ATP + (phosphate)n | - |
Mycobacterium tuberculosis | ADP + (phosphate)n+1 | - |
r | |
dADP + (phosphate)20 | - |
Mycobacterium tuberculosis | dATP + (phosphate)19 | - |
r | |
GDP + (phosphate)20 | isoform PPK1 affinity for the ADP as a substrate is about 70fold high as opposed to GDP | Mycobacterium tuberculosis | GTP + (phosphate)19 | - |
r | |
UDP + (phosphate)20 | - |
Mycobacterium tuberculosis | UTP + (phosphate)19 | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 87000, isoform PPK1, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
polyphosphate kinase-1 | isoform | Mycobacterium tuberculosis |
polyphosphate kinase-2 | isoform | Mycobacterium tuberculosis |
PPK1 | isoform | Mycobacterium tuberculosis |
PPK2 | isoform | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
44.6 | - |
ATP | mutant H510Q of isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
45 | - |
ATP | mutant H480Q of isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
45.6 | - |
ATP | mutant H480A of isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
46.9 | - |
ATP | isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
55 | - |
ATP | mutant Y524A of isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
55.4 | - |
ATP | mutant H510A of isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
210 | - |
ADP | isoform PPK2, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
332 | - |
ADP | mutant H510A of isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
622 | - |
ADP | mutant H510Q of isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
645 | - |
GDP | isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
943 | - |
ADP | isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
950 | - |
ADP | mutant H480A of isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
971 | - |
ADP | mutant H480Q of isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
994 | - |
ADP | mutant Y524A of isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.2 | - |
ATP | mutant Y524A of isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
10.5 | - |
ATP | mutant H510A of isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
25.5 | - |
ADP | isoform PPK2, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
29 | - |
ATP | mutant H510Q of isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
31 | - |
ATP | mutant H480A of isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
32.6 | - |
ATP | mutant H480Q of isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
34.7 | - |
ATP | isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
49 | - |
ADP | mutant H510A of isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
57 | - |
GDP | isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
998 | - |
ADP | mutant Y524A of isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
3529 | - |
ADP | mutant H510Q of isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
4690 | - |
ADP | mutant H480A of isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
5222 | - |
ADP | mutant H480Q of isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
5743 | - |
ADP | isoform PPK1, pH and temperature not specified in the publication | Mycobacterium tuberculosis |