Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.4.1 extracted from

  • Shum, K.T.; Lui, E.L.; Wong, S.C.; Yeung, P.; Sam, L.; Wang, Y.; Watt, R.M.; Tanner, J.A.
    Aptamer-mediated inhibition of Mycobacterium tuberculosis polyphosphate kinase 2 (2011), Biochemistry, 50, 3261-3271 .
    View publication on PubMed
Search for more literature for 2.7.4.1

Application

Application Comment Organism
analysis enzyme-linked assay in which His-tagged PPK2 is immobilized on a plate and then biotinylated aptamer inhibitors are added Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
G-quadruplex thrombin binding aptamer binds to PPK2 with a KD of 870 nM, noncompetitive Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 (phosphate)45 pH 8.5, temperature not specified in the publication Mycobacterium tuberculosis
0.0054
-
 (phosphate)65 pH 8.5, temperature not specified in the publication Mycobacterium tuberculosis
0.006
-
 (phosphate)66 pH 8.5, temperature not specified in the publication Mycobacterium tuberculosis
0.0063
-
 (phosphate)46 pH 8.5, temperature not specified in the publication Mycobacterium tuberculosis
0.33
-
 ATP pH 8.5, temperature not specified in the publication Mycobacterium tuberculosis
0.38
-
 ADP pH 8.5, temperature not specified in the publication Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis O05877
-
-
Mycobacterium tuberculosis ATCC 25618 O05877
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + (phosphate)46
-
 Mycobacterium tuberculosis ATP + (phosphate)45
-
 r
ADP + (phosphate)46
-
 Mycobacterium tuberculosis ATCC 25618 ATP + (phosphate)45
-
 r
ADP + (phosphate)66
-
 Mycobacterium tuberculosis ATP + (phosphate)65
-
 r
ADP + (phosphate)66
-
 Mycobacterium tuberculosis ATCC 25618 ATP + (phosphate)65
-
 r
ATP + (phosphate)45
-
 Mycobacterium tuberculosis ADP + (phosphate)46
-
 r
ATP + (phosphate)45
-
 Mycobacterium tuberculosis ATCC 25618 ADP + (phosphate)46
-
 r
ATP + (phosphate)65
-
 Mycobacterium tuberculosis ADP + (phosphate)66
-
 r
ATP + (phosphate)65
-
 Mycobacterium tuberculosis ATCC 25618 ADP + (phosphate)66
-
 r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
 (phosphate)65 pH 8.5, temperature not specified in the publication Mycobacterium tuberculosis
0.02
-
 (phosphate)45 pH 8.5, temperature not specified in the publication Mycobacterium tuberculosis
0.05
-
 ATP pH 8.5, temperature not specified in the publication Mycobacterium tuberculosis
41.9
-
 ADP pH 8.5, temperature not specified in the publication Mycobacterium tuberculosis
50
-
 (phosphate)66 pH 8.5, temperature not specified in the publication Mycobacterium tuberculosis
53
-
 (phosphate)46 pH 8.5, temperature not specified in the publication Mycobacterium tuberculosis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000039
-
 pH 8.5, temperature not specified in the publication Mycobacterium tuberculosis G-quadruplex thrombin binding aptamer

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.15
-
 ATP pH 8.5, temperature not specified in the publication Mycobacterium tuberculosis
3.7
-
 (phosphate)65 pH 8.5, temperature not specified in the publication Mycobacterium tuberculosis
4
-
 (phosphate)45 pH 8.5, temperature not specified in the publication Mycobacterium tuberculosis
110
-
 ADP pH 8.5, temperature not specified in the publication Mycobacterium tuberculosis
8300
-
 (phosphate)66 pH 8.5, temperature not specified in the publication Mycobacterium tuberculosis
8400
-
 (phosphate)46 pH 8.5, temperature not specified in the publication Mycobacterium tuberculosis